Human JAK/STAT Pathway Phosphorylation Array (12 targets)

Product Sku:	SARB0054
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	12
Gene Symbols:	EGFR, JAK1, JAK2, PTPN11, PTPN6, SRC, STAT1, STAT2, STAT3, STAT5A\|STAT6, TYK2
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	Akt Signaling / HER/ErbB Signaling / JAK/STAT Signaling / MAPK Signaling / Notch Signaling / PI3K-AKT Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
EGFR (P-Ser1070) Gene Symbol: EGFR ERBB ERBB1 HER1 Gene ID: 1956 Accession #: P00533	JAK1 (P-Tyr1022) Gene Symbol: JAK1 JAK1A JAK1B Gene ID: 3716 Accession #: P23458	JAK2 (P-Tyr1007/1008) Gene Symbol: JAK2 Gene ID: 3717 Accession #: O60674	SHP-1 (P-Ser591) Gene Symbol: PTPN6 HCP PTP1C Gene ID: 5777 Accession #: P29350	SHP2 (P-Tyr542) Gene Symbol: PTPN11 PTP2C SHPTP2 Gene ID: 5781 Accession #: Q06124
Src (P-Tyr419) Gene Symbol: SRC SRC1 Gene ID: 6714 Accession #: P12931	STAT1 (P-Ser727) Gene Symbol: STAT1 Gene ID: 6772 Accession #: P42224	STAT2 (P-Tyr689) Gene Symbol: STAT2 Gene ID: 6773 Accession #: P52630	STAT3 (P-Tyr705) Gene Symbol: STAT3 APRF Gene ID: 6774 Accession #: P40763	STAT5 (P-Tyr694) Gene Symbol: STAT5A STAT5 Gene ID: 6776 Accession #: P42229
STAT6 (P-Tyr641) Gene Symbol: STAT6 Gene ID: 6778 Accession #: P42226	TYK2 (P-Tyr1054) Gene Symbol: TYK2 Gene ID: 7297 Accession #: P29597

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated anti-IgG
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis