Human NF-kB Pathway Phosphorylation Array (11 targets) (SARB0059)

SKU:
SARB0059
€469 - €912

Description

Human NF-kB Pathway Phosphorylation Array Product Information

Product Sku: SARB0059
Size: 2, 4, or 8
Species Detected: Human
Number of Targets Detected: 11
Gene Symbols: ATM, EIF2S1, HDAC2, HDAC4, MAP3K7, NFKBIA, RELA, RPS6KA5, STAT1, TBK1|ZAP70
Compatible Sample Types: Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle: Sandwich-based
Method of Detection: Chemiluminescence
Pathway: MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PKC Signaling
Quantitative/Semi-Quantitative: Semi-Quantitative
Solid Support: Membrane
Suggested Application(s): Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability: For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human NF-kB Pathway Phosphorylation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

ATM (P-Ser1981)
  • Gene Symbol: ATM
  • Gene ID: 472;
  • Accession #: Q13315
eIF-2a (P-Ser52)
  • Gene Symbol: EIF2S1 EIF2A
  • Gene ID: 1965;
  • Accession #: P05198
HDAC2 (P-Ser394)
  • Gene Symbol: HDAC2
  • Gene ID: 3066
  • Accession #: Q92769
HDAC4 (P-Ser632)
  • Gene Symbol: HDAC4 KIAA0288
  • Gene ID: 9759;
  • Accession #: P56524
IKB-alpha (P-Ser32)
  • Gene Symbol: NFKBIA IKBA MAD3 NFKBI
  • Gene ID: 4792;
  • Accession #: P25963
MSK1 (P-Ser376)
  • Gene Symbol: RPS6KA5 MSK1
  • Gene ID: 9252;
  • Accession #: O75582
NF-kB (P-Ser536)
  • Gene Symbol: RELA NFKB3
  • Gene ID: 5970;
  • Accession #: Q04206
STAT1 (P-Ser727)
  • Gene Symbol: STAT1
  • Gene ID: 6772;
  • Accession #: P42224
TAK1 (P-Ser412)
  • Gene Symbol: MAP3K7 TAK1
  • Gene ID: 6885;
  • Accession #: O43318
TBK1 (P-Ser172)
  • Gene Symbol: TBK1 NAK
  • Gene ID: 29110;
  • Accession #: Q9UHD2
ZAP70 (P-Tyr292)
  • Gene Symbol: ZAP70 SRK
  • Gene ID: 7535;
  • Accession #: P43403
       

Human NF-kB Pathway Phosphorylation Array Kit Components

  • Human NF-kB Pathway Phosphorylation Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or plastic Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.

Human NF-kB Pathway Phosphorylation Array Protocol Outline

1. Block membranes
2. Incubate with Sample
3. Incubate with Detection Antibody Cocktail
4. Incubate with HRP-Conjugated anti-IgG
5. Incubate with Detection Buffers
6. Image with chemiluminescent imaging system
7. Perform densitometry and analysis
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Additional Information

Product type:
Phosphorylation Array
Target:
ATM (P-Ser1981)
Target:
eIF-2a (P-Ser52)
Target:
HDAC2 (P-Ser394)
Target:
HDAC4 (P-Ser632)
Target:
IKB-alpha (P-Ser32)
Target:
MSK1 (P-Ser376)
Target:
NF-kB (P-Ser536)
Target:
STAT1 (P-Ser727)
Target:
TAK1 (P-Ser412)
Target:
TBK1 (P-Ser172)
Target:
ZAP70 (P-Tyr292)
Reactivity:
Human
Application:
Multiplex Array
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