Human NF-kB Pathway Phosphorylation Array (11 targets) (SARB0059)
- SKU:
- SARB0059
- Product Type:
- Phosphorylation Array
- Analytes:
- ATM (P-Ser1981)
- eIF-2a (P-Ser52)
- HDAC2 (P-Ser394)
- HDAC4 (P-Ser632)
- IKB-alpha (P-Ser32)
- MSK1 (P-Ser376)
- NF-kB (P-Ser536)
- STAT1 (P-Ser727)
- TAK1 (P-Ser412)
- TBK1 (P-Ser172)
- ZAP70 (P-Tyr292)
- Reactivity:
- Human
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
| Product Sku: | SARB0059 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 11 |
| Gene Symbols: | ATM, EIF2S1, HDAC2, HDAC4, MAP3K7, NFKBIA, RELA, RPS6KA5, STAT1, TBK1|ZAP70 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PKC Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
ATM (P-Ser1981)
| eIF-2a (P-Ser52)
| HDAC2 (P-Ser394)
| HDAC4 (P-Ser632)
| IKB-alpha (P-Ser32)
|
MSK1 (P-Ser376)
| NF-kB (P-Ser536)
| STAT1 (P-Ser727)
| TAK1 (P-Ser412)
| TBK1 (P-Ser172)
|
ZAP70 (P-Tyr292)
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human NF-kB Pathway Phosphorylation Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit-IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or plastic Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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