Human/Mouse MAPK Phosphorylation Array (17 targets) (SARB0055)
- SKU:
- SARB0055
- Product Type:
- Phosphorylation Array
- Analytes:
- Akt (P-Ser473) (PKB (P-Ser473))
- CREB (P-Ser133)
- ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
- GSK3a (P-Ser21)
- GSK3b (P-Ser9)
- HSP27 (P-Ser82)
- JNK (P-Thr183)
- MEK (P-Ser217/Ser221)
- MKK3 (P-Ser189)
- MKK6 (P-Ser207)
- MSK2 (P-Ser360)
- mTOR (P-Ser2448)
- p38 (P-Thr180/Tyr182)
- P53 (P-Ser15)
- P70S6K (P-Thr421/Ser424)
- RSK1 (P-Ser380)
- RSK2 (P-Ser386)
- Reactivity:
- Human
- Mouse
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
| Product Sku: | SARB0055 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human / Mouse |
| Number of Targets Detected: | 17 |
| Gene Symbols: | AKT1, CREB1, GSK3A, GSK3B, HSPB1, MAP2K1, MAP2K3, MAP2K6, MAPK1, MAPK14|MAPK8, MTOR, RPS6KA1, RPS6KA3, RPS6KA4, RPS6KB1, TP53 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / HER/ErbB Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / NFkB Signaling / PI3K-AKT Signaling / Wnt/beta-Catenin Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Akt (P-Ser473) (PKB (P-Ser473))
| CREB (P-Ser133)
| ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
| GSK3a (P-Ser21)
| GSK3b (P-Ser9)
|
HSP27 (P-Ser82)
| JNK (P-Thr183)
| MEK (P-Ser217/Ser221)
| MKK3 (P-Ser189)
| MKK6 (P-Ser207)
|
MSK2 (P-Ser360)
| mTOR (P-Ser2448)
| p38 (P-Thr180/Tyr182)
| P53 (P-Ser15)
| P70S6K (P-Thr421/Ser424)
|
RSK1 (P-Ser380)
| RSK2 (P-Ser386)
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit-IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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