Human/Mouse MAPK Phosphorylation Array (17 targets) (SARB0055)

SKU:
SARB0055
€523 - €1,030

Description

Human/Mouse MAPK Phosphorylation Array Product Information

Product Sku:SARB0055
Size:2, 4, or 8
Species Detected:Human / Mouse
Number of Targets Detected:17
Gene Symbols:AKT1, CREB1, GSK3A, GSK3B, HSPB1, MAP2K1, MAP2K3, MAP2K6, MAPK1, MAPK14|MAPK8, MTOR, RPS6KA1, RPS6KA3, RPS6KA4, RPS6KB1, TP53
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / HER/ErbB Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / NFkB Signaling / PI3K-AKT Signaling / Wnt/beta-Catenin Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human/Mouse MAPK Phosphorylation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Akt (P-Ser473) (PKB (P-Ser473))
  • Gene Symbol: AKT1 PKB RAC
  • Gene ID: 207
  • Accession #: P31749
CREB (P-Ser133)
  • Gene Symbol: CREB1
  • Gene ID: 1385
  • Accession #: P16220
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
  • Gene Symbol: MAPK1 ERK2 PRKM1 PRKM2
  • Gene ID: 5594
  • Accession #: P28482
GSK3a (P-Ser21)
  • Gene Symbol: GSK3A
  • Gene ID: 2931
  • Accession #: P49840
GSK3b (P-Ser9)
  • Gene Symbol: GSK3B
  • Gene ID: 2932
  • Accession #: P49841
HSP27 (P-Ser82)
  • Gene Symbol: HSPB1 HSP27 HSP28
  • Gene ID: 3315
  • Accession #: P04792
JNK (P-Thr183)
  • Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C
  • Gene ID: 5599
  • Accession #: P45983
MEK (P-Ser217/Ser221)
  • Gene Symbol: MAP2K1 MEK1 PRKMK1
  • Gene ID: 5604
  • Accession #: Q02750
MKK3 (P-Ser189)
  • Gene Symbol: MAP2K3 MEK3 MKK3 PRKMK3 SKK2
  • Gene ID: 5606
  • Accession #: P46734
MKK6 (P-Ser207)
  • Gene Symbol: MAP2K6 MEK6 MKK6 PRKMK6 SKK3
  • Gene ID: 5608
  • Accession #: P52564
MSK2 (P-Ser360)
  • Gene Symbol: RPS6KA4 MSK2
  • Gene ID: 8986
  • Accession #: O75676
mTOR (P-Ser2448)
  • Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1
  • Gene ID: 2475
  • Accession #: P42345
p38 (P-Thr180/Tyr182)
  • Gene Symbol: MAPK14 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A
  • Gene ID: 1432
  • Accession #: Q16539
  

Human/Mouse MAPK Phosphorylation Array Kit Components

  • Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human/Mouse MAPK Phosphorylation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Phosphorylation Array
Target:
Akt (P-Ser473) (PKB (P-Ser473))
Target:
CREB (P-Ser133)
Target:
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
Target:
GSK3a (P-Ser21)
Target:
GSK3b (P-Ser9)
Target:
HSP27 (P-Ser82)
Target:
JNK (P-Thr183)
Target:
MEK (P-Ser217/Ser221)
Target:
MKK3 (P-Ser189)
Target:
MKK6 (P-Ser207)
Target:
MSK2 (P-Ser360)
Target:
mTOR (P-Ser2448)
Target:
p38 (P-Thr180/Tyr182)
Target:
P53 (P-Ser15)
Target:
P70S6K (P-Thr421/Ser424)
Target:
RSK1 (P-Ser380)
Target:
RSK2 (P-Ser386)
Reactivity:
Human
Reactivity:
Mouse
Application:
Multiplex Array
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