Human EGFR Phosphorylation Array (16 targets)

Product Sku:	SARB0062
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	16
Gene Symbols:	EGFR, ERBB2, ERBB3, ERBB4
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	HER/ErbB Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
EGFR (P-Tyr845) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR (P-Tyr992) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR (P-Tyr1045) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR (P-Tyr1068) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR (P-Tyr1086) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533
EGFR (P-Tyr1148) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR (P-Tyr1173) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR (P-Ser1046/1047) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	ErbB2 (P-Tyr877) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2 (P-Tyr1112) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626
ErbB2 (P-Tyr 1221/1222) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2 (P-Tyr1248) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2 (P-Thr686) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2 (P-Ser1113) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB3 (P-Tyr1289) Gene Symbol: ErbB3 Gene ID: 2065 Accession #: P21860
ErbB4 (P-Tyr1284) Gene Symbol: ErbB4 Gene ID: 2066 Accession #: Q15303

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis