Human EGFR Phosphorylation Array (16 targets) (SARB0062)

SKU:
SARB0062
€486 - €948

Description

Human EGFR Phosphorylation Array Product Information

Product Sku:SARB0062
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:16
Gene Symbols:EGFR, ERBB2, ERBB3, ERBB4
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:HER/ErbB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human EGFR Phosphorylation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

EGFR (P-Tyr845)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR (P-Tyr992)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR (P-Tyr1045)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR (P-Tyr1068)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR (P-Tyr1086)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR (P-Tyr1148)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR (P-Tyr1173)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR (P-Ser1046/1047)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
ErbB2 (P-Tyr877)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2 (P-Tyr1112)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2 (P-Tyr 1221/1222)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2 (P-Tyr1248)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2 (P-Thr686)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
  

Human EGFR Phosphorylation Array Kit Components

  • Human EGFR Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail Set I
  • Phosphatase Inhibitor Cocktail II
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human EGFR Phosphorylation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Phosphorylation Array
Target:
EGFR (P-Tyr845)
Target:
EGFR (P-Tyr992)
Target:
EGFR (P-Tyr1045)
Target:
EGFR (P-Tyr1068)
Target:
EGFR (P-Tyr1086)
Target:
EGFR (P-Tyr1148)
Target:
EGFR (P-Tyr1173)
Target:
EGFR (P-Ser1046/1047)
Target:
ErbB2 (P-Tyr877)
Target:
ErbB2 (P-Tyr1112)
Target:
ErbB2 (P-Tyr 1221/1222)
Target:
ErbB2 (P-Tyr1248)
Target:
ErbB2 (P-Thr686)
Target:
ErbB2 (P-Ser1113)
Target:
ErbB3 (P-Tyr1289)
Target:
ErbB4 (P-Tyr1284)
Reactivity:
Human
Application:
Multiplex Array
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