Description
Human EGFR Phosphorylation Array (16 targets) (SARB0062)
The Human EGFR Phosphorylation Array (SARB0062) is a powerful tool for studying the phosphorylation status of 16 different targets related to the epidermal growth factor receptor (EGFR) pathway. This array allows for the simultaneous detection and analysis of multiple phosphorylation events, providing valuable insights into signaling cascades and cellular responses.Each target on the array is carefully selected to provide comprehensive coverage of key components involved in EGFR signaling, including downstream effectors and regulators. The array is highly sensitive and specific, enabling the detection of subtle changes in phosphorylation levels that may be indicative of pathway activation or inhibition.Researchers can use the Human EGFR Phosphorylation Array to profile the phosphorylation status of multiple targets in a single experiment, saving time and resources compared to traditional methods.
This array is suitable for use with human samples, making it an invaluable tool for studying EGFR signaling in various physiological and pathological conditions.Overall, the Human EGFR Phosphorylation Array (SARB0062) is a versatile and reliable platform for investigating the complex network of signaling events associated with EGFR activation. Whether studying cancer, inflammation, or other disease processes, this array provides a comprehensive view of EGFR pathway activity, opening up new possibilities for research and drug development.
| Product Sku: | SARB0062 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 16 |
| Gene Symbols: | EGFR, ERBB2, ERBB3, ERBB4 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | HER/ErbB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
EGFR (P-Tyr845)
| EGFR (P-Tyr992)
| EGFR (P-Tyr1045)
| EGFR (P-Tyr1068)
| EGFR (P-Tyr1086)
|
EGFR (P-Tyr1148)
| EGFR (P-Tyr1173)
| EGFR (P-Ser1046/1047)
| ErbB2 (P-Tyr877)
| ErbB2 (P-Tyr1112)
|
ErbB2 (P-Tyr 1221/1222)
| ErbB2 (P-Tyr1248)
| ErbB2 (P-Thr686)
| ErbB2 (P-Ser1113)
| ErbB3 (P-Tyr1289)
|
ErbB4 (P-Tyr1284)
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human EGFR Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail Set I
- Phosphatase Inhibitor Cocktail II
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
