Human RTK Phosphorylation Array (71 targets) (SARB0064)

SKU:
SARB0064
€567 - €1,127

Description

Human RTK Phosphorylation Array Product Information

Product Sku:SARB0064
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:71
Gene Symbols:ABL1, AXL, BLK, BMX, BTK, CSF1R, CSK, DDR2, EGFR, EPHA1|EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, EPHB6, ERBB2, ERBB3, ERBB4, FER, FGFR1, FGFR2, FGR, FLT4, FRK, FYN, HCK, IGF1R, INSR, ITK, JAK1, JAK2, JAK3, KDR, KIT, LCK, LTK, LYN, MATK, MET, MUSK, NGFR, NTRK2, PDGFRA, PDGFRB, PTK2, PTK2B, RET, ROR1, ROR2, ROS1, RYK, SLPI, SRMS, SYK, TEC, TEK, TIE1, TNK1, TNK2, TXK, TYK2, TYRO3, ZAP70
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:PI3K-AKT Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human RTK Phosphorylation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

ABL1ACKALK-1AxlBlk
BMXBtkCskDtkEGFR
EphA1EphA2EphA3EphA4EphA5
EphA6EphA7EphA8EphB1EphB2
EphB3EphB4EphB6ErbB2ErbB3
ErbB4FAKFERFGFR1FGFR2
FGFR2 alpha isoformFgrFRKFynHck
HGFRIGF-1 RInsulin R (CD220)ItkJAK1
JAK2JAK3LCKLTKLyn
MATKM-CSF RMUSKNGFR (TNFRSF16)PDGFRA
PDGFRBPYK2RETROR1ROR2
ROSRYKSCF R (CD117/c-kit)SRMSSYK
TecTie-1Tie-2TNK1TRKB
TXKTyk2TYRO10 (DDR2/TKT)VEGFR2VEGFR3
ZAP70    

Human RTK Phosphorylation Array Kit Components

  • Human RTK Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Anti-Phospho Tyrosine Detection Antibody
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer (1 x 5 ml)
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail II
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human RTK Phosphorylation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Phosphorylation Array
Target:
ABL1
Target:
ACK
Target:
ALK-1
Target:
Axl
Target:
Blk
Target:
BMX
Target:
Btk
Target:
Csk
Target:
Dtk
Target:
EGFR
Target:
EphA1
Target:
EphA2
Target:
EphA3
Target:
EphA4
Target:
EphA5
Target:
EphA6
Target:
EphA7
Target:
EphA8
Target:
EphB1
Target:
EphB2
Target:
EphB3
Target:
EphB4
Target:
EphB6
Target:
ErbB2
Target:
ErbB3
Target:
ErbB4
Target:
FAK
Target:
FER
Target:
FGFR1
Target:
FGFR2
Target:
FGFR2 alpha isoform
Target:
Fgr
Target:
FRK
Target:
Fyn
Target:
Hck
Target:
HGFR
Target:
IGF-1 R
Target:
Insulin R (CD220)
Target:
Itk
Target:
JAK1
Target:
JAK2
Target:
JAK3
Target:
LCK
Target:
LTK
Reactivity:
Human
Application:
Multiplex Array
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