Human RTK Phosphorylation Array (71 targets) (SARB0064)
- SKU:
- SARB0064
- Product Type:
- Phosphorylation Array
- Analytes:
- ABL1
- ACK
- ALK-1
- Axl
- Blk
- BMX
- Btk
- Csk
- Dtk
- EGFR
- EphA1
- EphA2
- EphA3
- EphA4
- EphA5
- EphA6
- EphA7
- EphA8
- EphB1
- EphB2
- EphB3
- EphB4
- EphB6
- ErbB2
- ErbB3
- ErbB4
- FAK
- FER
- FGFR1
- FGFR2
- FGFR2 alpha isoform
- Fgr
- FRK
- Fyn
- Hck
- HGFR
- IGF-1 R
- Insulin R (CD220)
- Itk
- JAK1
- JAK2
- JAK3
- LCK
- LTK
- Reactivity:
- Human
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
Product Sku: | SARB0064 |
Size: | 2, 4, or 8 |
Species Detected: | Human |
Number of Targets Detected: | 71 |
Gene Symbols: | ABL1, AXL, BLK, BMX, BTK, CSF1R, CSK, DDR2, EGFR, EPHA1|EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, EPHB6, ERBB2, ERBB3, ERBB4, FER, FGFR1, FGFR2, FGR, FLT4, FRK, FYN, HCK, IGF1R, INSR, ITK, JAK1, JAK2, JAK3, KDR, KIT, LCK, LTK, LYN, MATK, MET, MUSK, NGFR, NTRK2, PDGFRA, PDGFRB, PTK2, PTK2B, RET, ROR1, ROR2, ROS1, RYK, SLPI, SRMS, SYK, TEC, TEK, TIE1, TNK1, TNK2, TXK, TYK2, TYRO3, ZAP70 |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | PI3K-AKT Signaling |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
ABL1
| ACK
| ALK-1
| Axl
| Blk
|
BMX
| Btk
| Csk
| Dtk
| EGFR
|
EphA1
| EphA2
| EphA3
| EphA4
| EphA5
|
EphA6
| EphA7
| EphA8
| EphB1
| EphB2
|
EphB3
| EphB4
| EphB6
| ErbB2
| ErbB3
|
ErbB4
| FAK
| FER
| FGFR1
| FGFR2
|
FGFR2 alpha isoform
| Fgr
| FRK
| Fyn
| Hck
|
HGFR
| IGF-1 R
| Insulin R (CD220)
| Itk
| JAK1
|
JAK2
| JAK3
| LCK
| LTK
| Lyn
|
MATK
| M-CSF R
| MUSK
| NGFR (TNFRSF16)
| PDGFRA
|
PDGFRB
| PYK2
| RET
| ROR1
| ROR2
|
ROS
| RYK
| SCF R (CD117/c-kit)
| SRMS
| SYK
|
Tec
| Tie-1
| Tie-2
| TNK1
| TRKB
|
TXK
| Tyk2
| TYRO10 (DDR2/TKT)
| VEGFR2
| VEGFR3
|
ZAP70
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human RTK Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Anti-Phospho Tyrosine Detection Antibody
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer (1 x 5 ml)
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail II
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Biotinylated Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated Streptavidin |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |