Human Apoptosis Array (43 targets) (SARB0021)
- SKU:
- SARB0021
- Product Type:
- Protein Array
- Analytes:
- bad
- bax
- bcl-2
- bcl-w
- BID
- BIM
- Caspase-3
- Caspase-8
- CD40 (TNFRSF5)
- CD40 Ligand (TNFSF5)
- cIAP-2
- Cytochrome C
- DR6 (TNFRSF21)
- Fas (TNFRSF6/Apo-1)
- Fas Ligand (TNFSF6)
- HSP27
- HSP60
- HSP70
- HTRA2
- IGF-1
- IGF-2
- IGFBP-1
- IGFBP-2
- IGFBP-3
- IGFBP-4
- IGFBP-5
- IGFBP-6
- IGF-1 R
- livin
- p21
- p27
- p53
- SMAC
- Survivin (BIRC5)
- TNF RI (TNFRSF1A)
- TNF RII (TNFRSF1B)
- TNF alpha
- TNF beta (TNFSF1B)
- TRAIL R1 (TNFRSF10A/DR4)
- TRAIL R2 (TNFRSF10B/DR5)
- TRAIL R3 (TNFRSF10C)
- TRAIL R4 (TNFRSF10D)
- XIAP
- Reactivity:
- Human
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
| Product Sku: | SARB0021 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 43 |
| Gene Symbols: | BAD, BAX, BCL2, BCL2L11, BCL2L2, BID, BIRC3, BIRC5, BIRC7, CASP3|CASP8, CD40, CD40LG, CDKN1A, CYCS, DIABLO, FAS, FASLG, HSPA14, HSPA4, HSPB1, HTRA2, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6, LTA, SSSCA1, TNF, TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, TNFRSF1A, TNFRSF1B, TNFRSF21, TP53, XIAP |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / IGF Signaling / p53 Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
bad
| bax
| bcl-2
| bcl-w
| BID
|
BIM
| Caspase-3
| Caspase-8
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
|
cIAP-2
| Cytochrome C
| DR6 (TNFRSF21)
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
HSP27
| HSP60
| HSP70
| HTRA2
| IGF-1
|
IGF-2
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
IGFBP-5
| IGFBP-6
| IGF-1 R
| livin
| p21
|
p27
| p53
| SMAC
| Survivin (BIRC5)
| TNF RI (TNFRSF1A)
|
TNF RII (TNFRSF1B)
| TNF alpha
| TNF beta (TNFSF1B)
| TRAIL R1 (TNFRSF10A/DR4)
| TRAIL R2 (TNFRSF10B/DR5)
|
TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| XIAP
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Apoptosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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