Mouse Cytokine Array (34 targets) (SARB0082)

SKU:
SARB0082
€616 - €1,234

Description

Mouse Cytokine Array Product Information

Product Sku:SARB0082
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:34
Gene Symbols:CCL22, CXCL11, CXCL15, DPP4, FCGR2B, FGF2, FIGF, FLT1, FLT3LG, FLT4|ICAM1, IGF1, IGF2, IGFBP2, IL15, IL17RB, IL7, KDR, MET, MMP2, MMP3, MMP9, PPBP, RETN, SELE, SHH, SPP1, TIMP2, TNFRSF11B, TNFRSF18, TNFRSF19, TNFSF11, TSLP, TYRO3
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

bFGFCD26 (DPPIV)DtkE-SelectinFc gamma RIIB (CD32b)
Flt-3 LigandGITR (TNFRSF18)HGFRICAM-1 (CD54)IGFBP-2
IGF-1IGF-2IL-15IL-17 RBIL-7
I-TAC (CXCL11)Lungkine (CXCL15)MDC (CCL22)MMP-2MMP-3
Osteopontin (SPP1)Osteoprotegerin (TNFRSF11B)Pro-MMP-9ResistinSonic Hedgehog N-Terminal (Shh-N)
TCK-1 (CXCL7)TIMP-2TRANCE (TNFSF11)TROY (TNFRSF19)TSLP
VEGFR1VEGFR2VEGFR3VEGF-D 

Mouse Cytokine Array Kit Components

  • Mouse Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
bFGF
Target:
CD26 (DPPIV)
Target:
Dtk
Target:
E-Selectin
Target:
Fc gamma RIIB (CD32b)
Target:
Flt-3 Ligand
Target:
GITR (TNFRSF18)
Target:
HGFR
Target:
ICAM-1 (CD54)
Target:
IGFBP-2
Target:
IGF-1
Target:
IGF-2
Target:
IL-15
Target:
IL-17 RB
Target:
IL-7
Target:
I-TAC (CXCL11)
Target:
Lungkine (CXCL15)
Target:
MDC (CCL22)
Target:
MMP-2
Target:
MMP-3
Target:
Osteopontin (SPP1)
Target:
Osteoprotegerin (TNFRSF11B)
Target:
Pro-MMP-9
Target:
Resistin
Target:
Sonic Hedgehog N-Terminal (Shh-N)
Target:
TCK-1 (CXCL7)
Target:
TIMP-2
Target:
TRANCE (TNFSF11)
Target:
TROY (TNFRSF19)
Target:
TSLP
Target:
VEGFR1
Target:
VEGFR2
Target:
VEGFR3
Target:
VEGF-D
Reactivity:
Mouse
Application:
Multiplex Array
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