Mouse Cytokine Array (96 targets) (SARB0077)

SKU:
SARB0077
€855 - €1,761

Description

Mouse Cytokine Array Product Information

Product Sku: SARB0077
Size: 2, 4, or 8
Species Detected: Mouse
Number of Targets Detected: 96
Gene Symbols: AXL, CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL22, CCL24|CCL25, CCL27, CCL3, CCL5, CCL9, CD40, CSF1, CSF2, CSF2RB, CSF3, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL15, CXCL16, CXCL2, CXCL5, CXCL9, DPP4, FASLG, FCGR2B, FGF2, FIGF, FLT1, FLT3LG, FLT4, ICAM1, IFNG, IGF1, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL10, IL12A, IL13, IL15, IL17A, IL17RB, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, IL9, KDR, KITLG, LEP, LEPR, MET, MMP2, MMP3, MMP9, PF4, PPBP, RETN, SELE, SELL, SELP, SHH, SPP1, THPO, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF18, TNFRSF19, TNFRSF8, TNFSF11, TNFSF8, TPO, TSLP, TYRO3, VCAM1, VEGFA, XCL1
Compatible Sample Types: Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle: Sandwich-based
Method of Detection: Chemiluminescence
Pathway:  
Quantitative/Semi-Quantitative: Semi-Quantitative
Solid Support: Membrane
Suggested Application(s): Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability: For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Axl bFGF BLC (CXCL13) CD30 Ligand (TNFSF8) CD30 (TNFRSF8)
CD40 (TNFRSF5) CRG-2 CTACK (CCL27) CXCL16 CD26 (DPPIV)
Dtk Eotaxin-1 (CCL11) Eotaxin-2 (MPIF-2/CCL24) E-Selectin Fas Ligand (TNFSF6)
Fc gamma RIIB (CD32b) Flt-3 Ligand Fractalkine (CX3CL1) GCSF GITR (TNFRSF18)
GM-CSF HGFR ICAM-1 (CD54) IFN-gamma IGFBP-2
IGFBP-3 IGFBP-5 IGFBP-6 IGF-1 IGF-2
IL-1 beta (IL-1 F2) IL-10 IL-12 p40/p70 IL12B IL-12 p70
IL-13 IL-15 IL-17A IL-17 RB IL-1 alpha (IL-1 F1)
IL-2 IL-3 IL-3 R beta IL-4 IL-5
IL-6 IL-7 IL-9 I-TAC (CXCL11) KC (CXCL1)
Leptin Leptin R LIX L-Selectin (CD62L) Lungkine (CXCL15)
Lymphotactin (XCL1) MCP-1 (CCL2) MCP-5 M-CSF MDC (CCL22)
MIG (CXCL9) MIP-1 alpha (CCL3) MIP-1 gamma MIP-2 MIP-3 beta (CCL19)
MIP-3 alpha (CCL20) MMP-2 MMP-3 Osteopontin (SPP1) Osteoprotegerin (TNFRSF11B)
Platelet Factor 4 (CXCL4) Pro-MMP-9 P-Selectin RANTES (CCL5) Resistin
SCF SDF-1 alpha (CXCL12 alpha) Sonic Hedgehog N-Terminal (Shh-N) TNF RI (TNFRSF1A) TNF RII (TNFRSF1B)
TARC (CCL17) I-309 (TCA-3/CCL1) TECK (CCL25) TCK-1 (CXCL7) TIMP-1
TIMP-2 TNF alpha Thrombopoietin (TPO) TRANCE (TNFSF11) TROY (TNFRSF19)
TSLP VCAM-1 (CD106) VEGF-A VEGFR1 VEGFR2
VEGFR3 VEGF-D      

Mouse Cytokine Array Kit Components

  • Mouse Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Cytokine Array Protocol Outline

1. Block membranes
2. Incubate with Sample
3. Incubate with Biotinylated Detection Antibody Cocktail
4. Incubate with HRP-Conjugated Streptavidin
5. Incubate with Detection Buffers
6. Image with chemiluminescent imaging system
7. Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Axl
Target:
bFGF
Target:
BLC (CXCL13)
Target:
CD30 Ligand (TNFSF8)
Target:
CD30 (TNFRSF8)
Target:
CD40 (TNFRSF5)
Target:
CRG-2
Target:
CTACK (CCL27)
Target:
CXCL16
Target:
CD26 (DPPIV)
Target:
Dtk
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
E-Selectin
Target:
Fas Ligand (TNFSF6)
Target:
Fc gamma RIIB (CD32b)
Target:
Flt-3 Ligand
Target:
Fractalkine (CX3CL1)
Target:
GCSF
Target:
GITR (TNFRSF18)
Target:
GM-CSF
Target:
HGFR
Target:
ICAM-1 (CD54)
Target:
IFN-gamma
Target:
IGFBP-2
Target:
IGFBP-3
Target:
IGFBP-5
Target:
IGFBP-6
Target:
IGF-1
Target:
IGF-2
Target:
IL-1 beta (IL-1 F2)
Target:
IL-10
Target:
IL-12 p40/p70
Target:
IL-12 p70
Target:
IL-13
Target:
IL-15
Target:
IL-17A
Target:
IL-17 RB
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-2
Target:
IL-3
Target:
IL-3 R beta
Target:
IL-4
Target:
IL-5
Reactivity:
Mouse
Application:
Multiplex Array
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