Mouse Cytokine Array (96 targets)

Product Sku:	SARB0077
Size:	2, 4, or 8
Species Detected:	Mouse
Number of Targets Detected:	96
Gene Symbols:	AXL, CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL22, CCL24\|CCL25, CCL27, CCL3, CCL5, CCL9, CD40, CSF1, CSF2, CSF2RB, CSF3, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL15, CXCL16, CXCL2, CXCL5, CXCL9, DPP4, FASLG, FCGR2B, FGF2, FIGF, FLT1, FLT3LG, FLT4, ICAM1, IFNG, IGF1, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL10, IL12A, IL13, IL15, IL17A, IL17RB, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, IL9, KDR, KITLG, LEP, LEPR, MET, MMP2, MMP3, MMP9, PF4, PPBP, RETN, SELE, SELL, SELP, SHH, SPP1, THPO, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF18, TNFRSF19, TNFRSF8, TNFSF11, TNFSF8, TPO, TSLP, TYRO3, VCAM1, VEGFA, XCL1
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
Axl Gene Symbol: Axl Gene ID: N/A Accession #: Q00993	bFGF Gene Symbol: FGF2 Gene ID: N/A Accession #: P15655	BLC (CXCL13) Gene Symbol: CXCL13 Gene ID: N/A Accession #: O55038	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Gene ID: N/A Accession #: P32972	CD30 (TNFRSF8) Gene Symbol: CD30 Gene ID: N/A Accession #: Q60846
CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: N/A Accession #: Q99NE2	CRG-2 Gene Symbol: Cxcl10 Gene ID: N/A Accession #: P17515	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: N/A Accession #: Q9Z1X0	CXCL16 Gene Symbol: CXCL16 Gene ID: N/A Accession #: Q5F2D5	CD26 (DPPIV) Gene Symbol: DPP4 Gene ID: N/A Accession #: P28843
Dtk Gene Symbol: TYRO3 Gene ID: N/A Accession #: O09080	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: N/A Accession #: P48298	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: N/A Accession #: Q9JKC0	E-Selectin Gene Symbol: SELE Gene ID: N/A Accession #: Q00690	Fas Ligand (TNFSF6) Gene Symbol: FASLG Gene ID: N/A Accession #: P41047
Fc gamma RIIB (CD32b) Gene Symbol: FCGR2B Gene ID: N/A Accession #: P08101	Flt-3 Ligand Gene Symbol: FLT3LG Gene ID: N/A Accession #: P49772	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Gene ID: N/A Accession #: O35188	GCSF Gene Symbol: CSF3 Gene ID: N/A Accession #: P09920	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Gene ID: N/A Accession #: Q9JKR2
GM-CSF Gene Symbol: CSF2 Gene ID: N/A Accession #: P26955	HGFR Gene Symbol: MET Gene ID: N/A Accession #: D3YVY2	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: N/A Accession #: P13597	IFN-gamma Gene Symbol: IFNG Gene ID: N/A Accession #: P01580	IGFBP-2 Gene Symbol: IGFBP2 Gene ID: N/A Accession #: P47877
IGFBP-3 Gene Symbol: IGFBP3 Gene ID: N/A Accession #: P47878	IGFBP-5 Gene Symbol: IGFBP5 Gene ID: N/A Accession #: Q07079	IGFBP-6 Gene Symbol: IGFBP6 Gene ID: N/A Accession #: Q91X24	IGF-1 Gene Symbol: IGF1 Gene ID: N/A Accession #: P05018	IGF-2 Gene Symbol: Igf2 Gene ID: N/A Accession #: P09535
IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: N/A Accession #: P10749	IL-10 Gene Symbol: IL10 Gene ID: N/A Accession #: Q0VBJ1	IL-12 p40/p70 Gene Symbol: IL12A; IL12B Gene ID: N/A Accession #: P43432	IL-12 p70 Gene Symbol: IL12A Gene ID: N/A Accession #: P43431	IL-13 Gene Symbol: IL13 Gene ID: N/A Accession #: P20109
IL-15 Gene Symbol: IL15 Gene ID: N/A Accession #: Q1AHQ7	IL-17A Gene Symbol: IL17A Gene ID: N/A Accession #: Q60971	IL-17 RB Gene Symbol: IL17RB Gene ID: N/A Accession #: Q9JIP3	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: N/A Accession #: P01582	IL-2 Gene Symbol: IL2 Gene ID: N/A Accession #: Q791T3
IL-3 Gene Symbol: IL3 Gene ID: N/A Accession #: P01586	IL-3 R beta Gene Symbol: Csf2rb Gene ID: N/A Accession #: P26955	IL-4 Gene Symbol: IL4 Gene ID: N/A Accession #: P07750	IL-5 Gene Symbol: IL5 Gene ID: N/A Accession #: P04401	IL-6 Gene Symbol: IL6 Gene ID: N/A Accession #: P08505
IL-7 Gene Symbol: IL7 Gene ID: N/A Accession #: P10168	IL-9 Gene Symbol: IL9 Gene ID: N/A Accession #: P15247	I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: N/A Accession #: Q9JHH5	KC (CXCL1) Gene Symbol: CXCL1 Gene ID: N/A Accession #: P12850	Leptin Gene Symbol: LEP Gene ID: N/A Accession #: P41160
Leptin R Gene Symbol: LEPR Gene ID: N/A Accession #: Q61215	LIX Gene Symbol: Cxcl5 Gene ID: N/A Accession #: P50228	L-Selectin (CD62L) Gene Symbol: SELL Gene ID: N/A Accession #: Q3TCF3	Lungkine (CXCL15) Gene Symbol: CXCL15 Gene ID: N/A Accession #: Q3UQ15	Lymphotactin (XCL1) Gene Symbol: XCL1 Gene ID: N/A Accession #: P47993
MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: N/A Accession #: Q9QYD7	MCP-5 Gene Symbol: Ccl12 Gene ID: N/A Accession #: Q9QYD6	M-CSF Gene Symbol: CSF1 Gene ID: N/A Accession #: P07141	MDC (CCL22) Gene Symbol: CCL22 Gene ID: N/A Accession #: O88430	MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: N/A Accession #: P18340
MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: N/A Accession #: P14096	MIP-1 gamma Gene Symbol: Ccl9 Gene ID: N/A Accession #: Q5QNW2	MIP-2 Gene Symbol: Cxcl2 Gene ID: N/A Accession #: P10889	MIP-3 beta (CCL19) Gene Symbol: CCL19 Gene ID: N/A Accession #: O70460	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: N/A Accession #: O89093
MMP-2 Gene Symbol: MMP2 Gene ID: N/A Accession #: P33434	MMP-3 Gene Symbol: MMP3 Gene ID: N/A Accession #: P28862	Osteopontin (SPP1) Gene Symbol: SPP1 Gene ID: N/A Accession #: P19008	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: N/A Accession #: O70202	Platelet Factor 4 (CXCL4) Gene Symbol: PF4, CXCL4, SCYB4 Gene ID: N/A Accession #: Q9Z126
Pro-MMP-9 Gene Symbol: Mmp9 Gene ID: N/A Accession #: Q80XI8	P-Selectin Gene Symbol: SELP Gene ID: N/A Accession #: Q01102	RANTES (CCL5) Gene Symbol: CCL5 Gene ID: N/A Accession #: P30882	Resistin Gene Symbol: RETN Gene ID: N/A Accession #: Q99P87	SCF Gene Symbol: KITLG Gene ID: N/A Accession #: P20826
SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: N/A Accession #: P40224	Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH Gene ID: N/A Accession #: N/A	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: N/A Accession #: P25118	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: N/A Accession #: O88734	TARC (CCL17) Gene Symbol: CCL17 Gene ID: N/A Accession #: Q9WUZ6
I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: N/A Accession #: P14098	TECK (CCL25) Gene Symbol: CCL25 Gene ID: N/A Accession #: O35903	TCK-1 (CXCL7) Gene Symbol: Ppbp Gene ID: N/A Accession #: Q9EQI5	TIMP-1 Gene Symbol: TIMP1 Gene ID: N/A Accession #: P12032	TIMP-2 Gene Symbol: TIMP2 Gene ID: N/A Accession #: Q6GXA8
TNF alpha Gene Symbol: TNF Gene ID: N/A Accession #: Q62326	Thrombopoietin (TPO) Gene Symbol: THPO Gene ID: N/A Accession #: P40226	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Gene ID: N/A Accession #: Q9R1Y0	TROY (TNFRSF19) Gene Symbol: TNFRSF19 Gene ID: N/A Accession #: Q80T13	TSLP Gene Symbol: TSLP Gene ID: N/A Accession #: Q9JIE6
VCAM-1 (CD106) Gene Symbol: VCAM1 Gene ID: N/A Accession #: P29533	VEGF-A Gene Symbol: VEGFA Gene ID: N/A Accession #: Q00731	VEGFR1 Gene Symbol: Flt1 Gene ID: N/A Accession #: Q61517	VEGFR2 Gene Symbol: KDR Gene ID: N/A Accession #: Q3UQZ6	VEGFR3 Gene Symbol: FLT4 Gene ID: N/A Accession #: P35917
VEGF-D Gene Symbol: FIGF Gene ID: N/A Accession #: A2AIH3

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Mouse Cytokine Antibody Array C1000 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis