Mouse Cytokine Array (32 targets) (SARB0080)
- SKU:
- SARB0080
- Product type:
- Protein Array
- Target:
- 6Ckine (CCL21)
- Target:
- CTACK (CCL27)
- Target:
- Eotaxin-1 (CCL11)
- Target:
- GCSF
- Target:
- GM-CSF
- Target:
- IL-2
- Target:
- IL-3
- Target:
- IL-4
- Target:
- IL-5
- Target:
- IL-6
- Target:
- IL-9
- Target:
- IL-10
- Target:
- IL-12 p40/p70
- Target:
- IL-12 p70
- Target:
- IL-13
- Target:
- IL-17A
- Target:
- IFN-gamma
- Target:
- KC (CXCL1)
- Target:
- Leptin
- Target:
- MCP-1 (CCL2)
- Target:
- MCP-5
- Target:
- MIP-1 alpha (CCL3)
- Target:
- MIP-2
- Target:
- MIP-3 beta (CCL19)
- Target:
- RANTES (CCL5)
- Target:
- SCF
- Target:
- TNF RI (TNFRSF1A)
- Target:
- TARC (CCL17)
- Target:
- TIMP-1
- Target:
- TNF alpha
- Target:
- Thrombopoietin (TPO)
- Target:
- VEGF-A
- Reactivity:
- Mouse
- Application:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
Product Sku: | SARB0080 |
Size: | 2, 4, or 8 |
Species Detected: | Mouse |
Number of Targets Detected: | 32 |
Gene Symbols: | CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL21, CCL27, CCL3, CCL5|CSF2, CSF3, CXCL1, CXCL2, IFNG, IL10, IL12A, IL13, IL2, IL3, IL4, IL5, IL6, IL9, KITLG, LEP, THPO, TIMP1, TNF, TNFRSF1A, TPO, VEGFA |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
6Ckine (CCL21)
| CTACK (CCL27)
| Eotaxin-1 (CCL11)
| GCSF
| GM-CSF
|
IL-2
| IL-3
| IL-4
| IL-5
| IL-6
|
IL-9
| IL-10
| IL-12 p40/p70
| IL-12 p70
| IL-13
|
IL-17A
| IFN-gamma
| KC (CXCL1)
| Leptin
| MCP-1 (CCL2)
|
MCP-5
| MIP-1 alpha (CCL3)
| MIP-2
| MIP-3 beta (CCL19)
| RANTES (CCL5)
|
SCF
| TNF RI (TNFRSF1A)
| TARC (CCL17)
| TIMP-1
| TNF alpha
|
Thrombopoietin (TPO)
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Biotinylated Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated Streptavidin |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |