Mouse Cytokine Array (32 targets) (SARB0080)

SKU:
SARB0080
€644 - €1,297

Description

Mouse Cytokine Array Product Information

Product Sku:SARB0080
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:32
Gene Symbols:CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL21, CCL27, CCL3, CCL5|CSF2, CSF3, CXCL1, CXCL2, IFNG, IL10, IL12A, IL13, IL2, IL3, IL4, IL5, IL6, IL9, KITLG, LEP, THPO, TIMP1, TNF, TNFRSF1A, TPO, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

6Ckine (CCL21)
  • Gene Symbol: CCL21
  • Accession #: P86792
CTACK (CCL27)
  • Gene Symbol: CCL27
  • Accession #: Q9Z1X0
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Accession #: P48298
GCSF
  • Gene Symbol: CSF3
  • Accession #: P09920
GM-CSF
  • Gene Symbol: CSF2
  • Accession #: P26955
IL-2
  • Gene Symbol: IL2
  • Accession #: Q791T3
IL-3
  • Gene Symbol: IL3
  • Accession #: P01586
IL-4
  • Gene Symbol: IL4
  • Accession #: P07750
IL-5
  • Gene Symbol: IL5
  • Accession #: P04401
IL-6
  • Gene Symbol: IL6
  • Accession #: P08505
IL-9
  • Gene Symbol: IL9
  • Accession #: P15247
IL-10
  • Gene Symbol: IL10
  • Accession #: Q0VBJ1
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Accession #: P43432
  

Mouse Cytokine Array Kit Components

  • Mouse Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
6Ckine (CCL21)
Target:
CTACK (CCL27)
Target:
Eotaxin-1 (CCL11)
Target:
GCSF
Target:
GM-CSF
Target:
IL-2
Target:
IL-3
Target:
IL-4
Target:
IL-5
Target:
IL-6
Target:
IL-9
Target:
IL-10
Target:
IL-12 p40/p70
Target:
IL-12 p70
Target:
IL-13
Target:
IL-17A
Target:
IFN-gamma
Target:
KC (CXCL1)
Target:
Leptin
Target:
MCP-1 (CCL2)
Target:
MCP-5
Target:
MIP-1 alpha (CCL3)
Target:
MIP-2
Target:
MIP-3 beta (CCL19)
Target:
RANTES (CCL5)
Target:
SCF
Target:
TNF RI (TNFRSF1A)
Target:
TARC (CCL17)
Target:
TIMP-1
Target:
TNF alpha
Target:
Thrombopoietin (TPO)
Target:
VEGF-A
Reactivity:
Mouse
Application:
Multiplex Array
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