Mouse Cytokine Array (97 targets) (SARB0084)

SKU:
SARB0084
€904 - €1,868

Description

Mouse Cytokine Array Product Information

Product Sku:SARB0084
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:97
Gene Symbols:AREG, AXL, CCL11, CCL17, CCL19, CCL2, CCL20, CCL21, CCL22, CCL24|CCL3, CCL5, CCL9, CD27, CD36, CD70, CDH1, CSF3, CTF1, CTLA4, CX3CL1, CXCL1, CXCL11, CXCL13, CXCL15, CXCL16, CXCL2, CXCL9, DCN, DKK1, EGF, EPGN, F11R, FASLG, FCGR2B, FIGF, FLT1, FLT3LG, FLT4, GAS6, GZMB, HGF, IFNG, IGF2, IGFBP5, IGFBP6, IL10, IL11, IL12A, IL12B, IL13, IL15, IL17A, IL17F, IL17RB, IL1A, IL1B, IL1RN, IL2, IL20, IL21, IL25, IL2RA, IL3, IL4, IL5, IL6, IL9, KITLG, LEP, LEPR, LGALS1, MADCAM1, MFGE8, MMP2, MMP9, PRL, SELE, SELL, SLPI, SPINT1, SPP1, THPO, TNF, TNFRSF11B, TNFRSF13B, TNFRSF18, TNFRSF19, TNFRSF8, TNFSF11, TNFSF12, TNFSF18, TNFSF8, TPO, VCAM1, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

6Ckine (CCL21)ALK-1AmphiregulinAxlBLC (CXCL13)
Cardiotrophin-1 (CT-1)CD27 (TNFRSF7)CD27 Ligand (TNFSF7)CD30 (TNFRSF8)CD30 Ligand (TNFSF8)
CD36 (SR-B3)CTLA-4 (CD152)CXCL16DecorinDKK-1
E-CadherinEGFEotaxin-1 (CCL11)Eotaxin-2 (MPIF-2/CCL24)Epigen
E-SelectinFas Ligand (TNFSF6)Fc gamma RIIB (CD32b)Flt-3 LigandFractalkine (CX3CL1)
Galectin-1Gas 6GCSFGITR (TNFRSF18)GITR Ligand (TNFSF18)
Granzyme BHAI-1HGFIFN-gammaIGFBP-5
IGFBP-6IGF-2IL-1 alpha (IL-1 F1)IL-1 beta (IL-1 F2)IL-1 ra (IL-1 F3)
IL-2IL-2 R alphaIL-3IL-4IL-5
IL-6IL-9IL-10IL-11IL-12 p40
IL-12 p70IL-13IL-15IL-17AIL-17 RB
IL-17E (IL-25)IL-17FIL-20IL-21I-TAC (CXCL11)
JAM-A (CD321/F11R)KC (CXCL1)LeptinLeptin RL-Selectin (CD62L)
Lungkine (CXCL15)MAdCAM-1MCP-1 (CCL2)MDC (CCL22)MFG-E8
MIG (CXCL9)MIP-1 alpha (CCL3)MIP-1 gammaMIP-2MIP-3 alpha (CCL20)
MIP-3 beta (CCL19)MMP-2Osteopontin (SPP1)Osteoprotegerin (TNFRSF11B)Prolactin
Pro-MMP-9RANTES (CCL5)SCFTNF RI (TNFRSF1A)TNF RII (TNFRSF1B)
TACI (TNFRSF13B)TARC (CCL17)TNF alphaThrombopoietin (TPO)TRANCE (TNFSF11)
TROY (TNFRSF19)TWEAK R (TNFRSF12)VCAM-1 (CD106)VEGF-AVEGFR1
VEGFR3VEGF-D   

Mouse Cytokine Array Kit Components

  • Mouse Cytokine Antibody Array C6 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 4-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
6Ckine (CCL21)
Target:
ALK-1
Target:
Amphiregulin
Target:
Axl
Target:
BLC (CXCL13)
Target:
Cardiotrophin-1 (CT-1)
Target:
CD27 (TNFRSF7)
Target:
CD27 Ligand (TNFSF7)
Target:
CD30 (TNFRSF8)
Target:
CD30 Ligand (TNFSF8)
Target:
CD36 (SR-B3)
Target:
CTLA-4 (CD152)
Target:
CXCL16
Target:
Decorin
Target:
DKK-1
Target:
E-Cadherin
Target:
EGF
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
Epigen
Target:
E-Selectin
Target:
Fas Ligand (TNFSF6)
Target:
Fc gamma RIIB (CD32b)
Target:
Flt-3 Ligand
Target:
Fractalkine (CX3CL1)
Target:
Galectin-1
Target:
Gas 6
Target:
GCSF
Target:
GITR (TNFRSF18)
Target:
GITR Ligand (TNFSF18)
Target:
Granzyme B
Target:
HAI-1
Target:
HGF
Target:
IFN-gamma
Target:
IGFBP-5
Target:
IGFBP-6
Target:
IGF-2
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-1 ra (IL-1 F3)
Target:
IL-2
Target:
IL-2 R alpha
Target:
IL-3
Target:
IL-4
Reactivity:
Mouse
Application:
Multiplex Array
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