Mouse Cytokine Array (40 targets) (SARB0085)

SKU:
SARB0085
€543 - €1,075

Description

Mouse Cytokine Array Product Information

Product Sku:SARB0085
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:40
Gene Symbols:AHSG, BTC, C5, CCL6, CD48, CD6, CD80, CDH3, CLU, CST3|CXCL15, DLL4, EDAR, ESM1, GPNMB, GRN, H60A, HAVCR1, IFNA11, IGDCC4, IL33, IL7R, KREMEN1, LCN2, MBL2, METRN, NOV, OLR1, PARN, PGF, POSTN, PRSS22, PRSS27, PRSS8, RARRES2, REN1, SPOCK3, TNFRSF13C, TNFSF10, TNFSF4
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

CD80 (B7-1)BAFF RBetacellulin (BTC)C5aCCL6
CD48 (SLAMF2)CD6ChemerinClusterinLungkine (CXCL15)
Cystatin CDANDLL4EDAREndocan
Fetuin AH60IL-33 (IL-1 F11)IL-7 R alphaKremen-1
LimitinLipocalin-2 (NGAL)LOX-1MarapsinMBL-2
MeteorinNopeNOV (CCN3)Osteoactivin (GPNMB)OX40 Ligand
P-CadherinPeriostinPLGF-2ProgranulinProstasin
Renin 1Testican 3 (SPOCK3)TIM-1 (KIM-1)TRAIL (TNFSF10)Tryptase epsilon

Mouse Cytokine Array Kit Components

  • Mouse Cytokine Array C7 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 4-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
CD80 (B7-1)
Target:
BAFF R
Target:
Betacellulin (BTC)
Target:
C5a
Target:
CCL6
Target:
CD48 (SLAMF2)
Target:
CD6
Target:
Chemerin
Target:
Clusterin
Target:
Lungkine (CXCL15)
Target:
Cystatin C
Target:
DAN
Target:
DLL4
Target:
EDAR
Target:
Endocan
Target:
Fetuin A
Target:
H60
Target:
IL-33 (IL-1 F11)
Target:
IL-7 R alpha
Target:
Kremen-1
Target:
Limitin
Target:
Lipocalin-2 (NGAL)
Target:
LOX-1
Target:
Marapsin
Target:
MBL-2
Target:
Meteorin
Target:
Nope
Target:
NOV (CCN3)
Target:
Osteoactivin (GPNMB)
Target:
OX40 Ligand
Target:
P-Cadherin
Target:
Periostin
Target:
PLGF-2
Target:
Progranulin
Target:
Prostasin
Target:
Renin 1
Target:
Testican 3 (SPOCK3)
Target:
TIM-1 (KIM-1)
Target:
TRAIL (TNFSF10)
Target:
Tryptase epsilon
Reactivity:
Mouse
Application:
Multiplex Array
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