Canine Cytokine Array (40 targets) (SARB0008)

SKU:
SARB0008
€722 - €1,450

Description

Canine Cytokine Array Product Information

Product Sku:SARB0008
Size:2, 4, or 8
Species Detected:Canine
Number of Targets Detected:40
Gene Symbols:C1QTNF1, CCL2, CCL4, CSF2, CXCL1, CXCL8, DCN, EGFR, EPOR, FGF7|HGF, IFNG, IL10, IL12B, IL17A, IL1B, IL2, IL6, KITLG, LGALS3, MET, PDGFB, TNFRSF1A, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Canine Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Cystatin CDecorinEGFRErbB3Erythropoietin
FGF-7 (KGF)Galectin-3GASP-1 (WFIKKNRP)GM-CSFGRO alpha (CXCL1)
HGFHGFRIFN-gammaIGFBP-2IL-1 alpha (IL-1 F1)
IL-1 beta (IL-1 F2)IL-1 Ra (IL-1 F3)IL-10IL-12 p40IL-13
IL-17AIL-2IL-21IL-6IL-8 (CXCL8)
MCP-1 (CCL2)MIP-1 beta (CCL4)MMP-8NCAM-1 (CD56)Nope
PDGF-BBRAGERANTES (CCL5)ResistinSCF
TGF alphaTNF alphaTNF RI (TNFRSF1A)Trappin-2VEGF-A

Canine Cytokine Array Kit Components

  • Canine Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Canine Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Cystatin C
Target:
Decorin
Target:
EGFR
Target:
ErbB3
Target:
Erythropoietin
Target:
FGF-7 (KGF)
Target:
Galectin-3
Target:
GASP-1 (WFIKKNRP)
Target:
GM-CSF
Target:
GRO alpha (CXCL1)
Target:
HGF
Target:
HGFR
Target:
IFN-gamma
Target:
IGFBP-2
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-1 Ra (IL-1 F3)
Target:
IL-10
Target:
IL-12 p40
Target:
IL-13
Target:
IL-17A
Target:
IL-2
Target:
IL-21
Target:
IL-6
Target:
IL-8 (CXCL8)
Target:
MCP-1 (CCL2)
Target:
MIP-1 beta (CCL4)
Target:
MMP-8
Target:
NCAM-1 (CD56)
Target:
Nope
Target:
PDGF-BB
Target:
RAGE
Target:
RANTES (CCL5)
Target:
Resistin
Target:
SCF
Target:
TGF alpha
Target:
TNF alpha
Target:
TNF RI (TNFRSF1A)
Target:
Trappin-2
Target:
VEGF-A
Reactivity:
Canine
Application:
Multiplex Array
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