Human Cytokine Array (40 targets) (SARB0033)

SKU:
SARB0033
€888 - €1,832

Description

Human Cytokine Array Product Information

Product Sku:SARB0033
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:40
Gene Symbols:ADAM9, BMP2, C5, CD200, CD244, CD97, CTSL, FABP2, FAP, FGF19|FRZB, GDF2, GPNMB, IFNAR2, IGF2R, IL1RL2, IL24, IL33, KLK14, LGALS3, LGMN, MBL2, MET, MME, NOTCH1, NOV, OLR1, PDCD1, PGLYRP1, RARRES2, SERPINA4, TF, THBD, TLR2, TNFRSF6B, TNFSF13, WIF1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

2B4 (CD244)ADAM-9APRILBMP-2BMP-9
Cathepsin LCD200CD97ChemerinC5a
DcR3FABP2FAPFGF-19Galectin-3
HGFRIFN-alpha / beta R2IGF-2 RIL-1 R6 (IL-1 Rrp2)IL-24
IL-33 (IL-1 F11)Kallikrein 14LegumainLOX-1MBL
NeprilysinNotch-1NOV (CCN3)Osteoactivin (GPNMB)PD-1
PGRPsSERPINA4sFRP-3ThrombomodulinTLR2
TransferrinWIF-1   

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C11 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
2B4 (CD244)
Target:
ADAM-9
Target:
APRIL
Target:
BMP-2
Target:
BMP-9
Target:
Cathepsin L
Target:
CD200
Target:
CD97
Target:
Chemerin
Target:
C5a
Target:
DcR3
Target:
FABP2
Target:
FAP
Target:
FGF-19
Target:
Galectin-3
Target:
HGFR
Target:
IFN-alpha / beta R2
Target:
IGF-2 R
Target:
IL-1 R6 (IL-1 Rrp2)
Target:
IL-24
Target:
IL-33 (IL-1 F11)
Target:
Kallikrein 14
Target:
Legumain
Target:
LOX-1
Target:
MBL
Target:
Neprilysin
Target:
Notch-1
Target:
NOV (CCN3)
Target:
Osteoactivin (GPNMB)
Target:
PD-1
Target:
PGRPs
Target:
SERPINA4
Target:
sFRP-3
Target:
Thrombomodulin
Target:
TLR2
Target:
Transferrin
Target:
WIF-1
Reactivity:
Human
Application:
Multiplex Array
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