Description
Human T Cell Response Array (10 targets) (SARB0067)
The Human T Cell Response Array - 10 Targets (SARB0067) is a comprehensive research tool for studying T cell responses in humans. This array includes 10 targets related to T cell activation, cytokine production, and immune regulation, allowing researchers to analyze the complex interactions of the human immune system.With a focus on T cell function, this array provides valuable information on the molecular mechanisms that drive immune responses in various conditions, including infection, autoimmune diseases, and cancer. By profiling multiple targets simultaneously, researchers can gain a deeper understanding of the T cell response and identify potential therapeutic targets for immune-mediated diseases.Designed for high-throughput screening, the Human T Cell Response Array - 10 Targets (SARB0067) offers a cost-effective and efficient solution for studying T cell biology.
Whether studying T cell activation in response to stimuli or investigating cytokine production in disease states, this array provides valuable insights into the complex interplay of immune cells in the human body.Overall, the Human T Cell Response Array - 10 Targets (SARB0067) is a powerful tool for researchers looking to investigate T cell responses in human samples. By profiling multiple targets simultaneously, this array enables comprehensive analysis of T cell function and immune regulation, leading to a better understanding of immune-mediated diseases and potential therapeutic strategies.
| Product Sku: | SARB0067 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 10 |
| Gene Symbols: | IFNG, IL10, IL12A, IL17A, IL2, IL4, IL5, IL9, TGFB1, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
IL-2
| IL-4
| IL-5
| IL-9
| IL-10
|
IL-12 p70
| IL-17A
| IFN-gamma
| TGF beta 1
| TNF alpha
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human T Cell Response Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
