Bovine T Cell Response Array (10 targets) (SARB0006)

SKU:
SARB0006
€514 - €993

Description

Bovine T Cell Response Array Product Information

Product Sku:SARB0006
Size:2, 4, or 8
Species Detected:Bovine
Number of Targets Detected:10
Gene Symbols:CD40LG, IFNG, IL10, IL13, IL17A, IL1A, IL1B, IL21, NCAM1, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:MAPK Signaling / NFkB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Bovine T Cell Response Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

CD40 Ligand (TNFSF5)
  • Gene Symbol: CD40LG CD40L TNFSF5
  • Gene ID: 282387
  • Accession #: P51749
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 281237
  • Accession #: P07353
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 281250
  • Accession #: P08831
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 281251
  • Accession #: P09428
IL-10
  • Gene Symbol: IL10
  • Gene ID: 281246
  • Accession #: P43480
IL-13
  • Gene Symbol: IL13
  • Gene ID: 281247
  • Accession #: Q9XSV9
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 282863
  • Accession #: Q687Y7
IL-21
  • Gene Symbol: IL21
  • Gene ID: 378475
  • Accession #: Q76LU5
NCAM-1 (CD56)
  • Gene Symbol: NCAM1 NCAM
  • Gene ID: 281941
  • Accession #: P31836
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 280943
  • Accession #: Q06599

Bovine T Cell Response Array Kit Components

  • Bovine T Cell Response Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Bovine T Cell Response Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
CD40 Ligand (TNFSF5)
Target:
IFN-gamma
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-10
Target:
IL-13
Target:
IL-17A
Target:
IL-21
Target:
NCAM-1 (CD56)
Target:
TNF alpha
Reactivity:
Bovine
Application:
Multiplex Array
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