Bovine Cytokine Array (10 targets) (SARB0003)

SKU:
SARB0003
€514 - €993

Description

Bovine Cytokine Array Product Information

Product Sku:SARB0003
Size:2, 4, or 8
Species Detected:Bovine
Number of Targets Detected:10
Gene Symbols:CCL4, CXCL10, CXCL9, IFNA1, IFNG, IL13, IL1A, IL21, IL36RN, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Bovine Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

IFN alpha
  • Gene Symbol: IFNA1 IFNA13
  • Gene ID: 3439 3447
  • Accession #: P01562
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A IL1F1
  • Gene ID: 3552
  • Accession #: P01583
IL-36 Ra (IL-1 F5)
  • Gene Symbol: IL36RN FIL1D IL1F5 IL1HY1 IL1L1 IL1RP3 UNQ1896/PRO4342
  • Gene ID: 26525
  • Accession #: Q9UBH0
IL-13
  • Gene Symbol: IL13 NC30
  • Gene ID: 3596
  • Accession #: P35225
IL-21
  • Gene Symbol: IL21
  • Gene ID: 59067
  • Accession #: Q9HBE4
IP-10 (CXCL10)
  • Gene Symbol: CXCL10 INP10 SCYB10
  • Gene ID: 3627
  • Accession #: P02778
MIG (CXCL9)
  • Gene Symbol: CXCL9 CMK MIG SCYB9
  • Gene ID: 4283
  • Accession #: Q07325
MIP-1 beta (CCL4)
  • Gene Symbol: CCL4 LAG1 MIP1B SCYA4
  • Gene ID: 388372 6351
  • Accession #: P13236
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 7124
  • Accession #: P01375

Bovine Cytokine Array Kit Components

  • Bovine Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Bovine Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
IFN alpha
Target:
IFN-gamma
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-36 Ra (IL-1 F5)
Target:
IL-13
Target:
IL-21
Target:
IP-10 (CXCL10)
Target:
MIG (CXCL9)
Target:
MIP-1 beta (CCL4)
Target:
TNF alpha
Reactivity:
Bovine
Application:
Multiplex Array
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