Description
Equine Cytokine Array (10 targets) (SARB0013)
The Equine Cytokine Array 10 Targets (SARB0013) is a powerful research tool designed for studying cytokine expression in horses. This array allows for the simultaneous detection of 10 different cytokines, providing valuable insights into the immune response and inflammatory processes in equine species.With a focus on key cytokines involved in immune regulation, inflammation, and cell signaling, this array is ideal for researchers investigating the immune system of horses. By profiling cytokine levels in biological samples such as serum or tissue lysates, researchers can gain a comprehensive understanding of the immune response in horses under various conditions.
The Equine Cytokine Array 10 Targets (SARB0013) offers high sensitivity and specificity, making it a reliable tool for identifying changes in cytokine expression levels. By studying cytokine profiles in horse samples, researchers can uncover potential biomarkers for disease diagnosis, monitor immune responses to treatments, and advance knowledge in equine immunology.Overall, the Equine Cytokine Array 10 Targets (SARB0013) is a valuable resource for researchers looking to unravel the complexities of the equine immune system and pave the way for novel therapeutic interventions in veterinary medicine.
| Product Sku: | SARB0013 |
| Size: | 2, 4, or 8 |
| Species Detected: | Equine |
| Number of Targets Detected: | 10 |
| Gene Symbols: | CCL2, CXCL8, IFNG, IL10, IL1A, IL1RA, IL1RN, IL2, IL4, IL8|MCP1, SCYA2, VEGF, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 Ra (IL-1 F3)
| IL-2
| IL-4
|
IL-8 (CXCL8)
| IL-10
| IL-15
| MCP-1 (CCL2)
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Equine (Horse) Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
