Sheep Cytokine Array (10 targets) (SARB0093)

SKU:
SARB0093
€514 - €993

Description

Sheep Cytokine Array Product Information

Product Sku:SARB0093
Size:2, 4, or 8
Species Detected:Sheep
Number of Targets Detected:10
Gene Symbols:CXCL8, DCN, FRZB, IFNG, IL17A, IL21, IP-10, TNF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Sheep Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Decorin
  • Gene Symbol: DCN
  • Gene ID: 443048
  • Accession #: Q9TTE2
IFN-gamma
  • Gene Symbol: DCN
  • Gene ID: 443048
  • Accession #: Q9TTE2
IL-17A
  • Gene Symbol: IFNG
  • Gene ID: 443396
  • Accession #: P17773
IL-21
  • Gene Symbol: IL17A
  • Gene ID: 101103931
  • Accession #: W5PWW6
IL-8 (CXCL8)
  • Gene Symbol: IL21
  • Gene ID: 100862657
  • Accession #: H6SGV5
IP-10 (CXCL10)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 443418
  • Accession #: P36925
MIG (CXCL9)
  • Gene Symbol: IP-10 CXCL10
  • Gene ID: 442997
  • Accession #: Q95MZ7
sFRP-3
  • Accession #:
TNF alpha
  • Gene Symbol: FRZB
  • Gene ID: 101118419
  • Accession #: W5Q5L6
VEGF-A
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 443540
  • Accession #: P23383

Sheep Cytokine Array Kit Components

  • Sheep Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Sheep Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Decorin
Target:
IFN-gamma
Target:
IL-17A
Target:
IL-21
Target:
IL-8 (CXCL8)
Target:
IP-10 (CXCL10)
Target:
MIG (CXCL9)
Target:
sFRP-3
Target:
TNF alpha
Target:
VEGF-A
Reactivity:
Sheep
Application:
Multiplex Array
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