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Dolphin Cytokine Array (10 targets) (SARB0012)

SKU:
SARB0012
Product type:
Protein Array
Target:
IFN-gamma
Target:
IL-1 beta (IL-1 F2)
Target:
IL-1 Ra (IL-1 F3)
Target:
IL-2
Target:
IL-6
Target:
IL-8 (CXCL8)
Target:
IL-13
Target:
IL-17A
Target:
IP-10 (CXCL10)
Target:
TNF alpha
Reactivity:
Dolphin
Application:
Multiplex Array
€514 - €993
Frequently bought together:

Description

system_update_altDatasheet
Product Sku:SARB0012
Size:2, 4, or 8
Species Detected:Dolphin
Number of Targets Detected:10
Gene Symbols:CXCL8, IFNG, IL13, IL1RA, IL1RN, IL2, IL6, IL8, TNF, TNFA|TNFSF2
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 101337436
  • Accession #: Q9TV67
IL-1 beta (IL-1 F2)
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #:
IL-1 Ra (IL-1 F3)
  • Gene Symbol: IL1RA IL1RN
  • Gene ID: 101328434
  • Accession #: Q9GMZ4
IL-2
  • Gene Symbol: IL2
  • Gene ID: N/A
  • Accession #: B3V280
IL-6
  • Gene Symbol: IL6
  • Gene ID: N/A
  • Accession #:
IL-8 (CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 101331085
  • Accession #: Q7YRB5
IL-13
  • Gene Symbol: IL13
  • Gene ID: N/A
  • Accession #: B3V281
IL-17A
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #:
IP-10 (CXCL10)
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #:
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 101318178
  • Accession #: Q9BEA1
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Dolphin Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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