Description
Dolphin Cytokine Array (10 targets) (SARB0012)
The Dolphin Cytokine Array 10 Targets (SARB0012) is a powerful tool for researchers studying cytokine expression and activity in a variety of biological samples. This array covers 10 key cytokines, allowing for comprehensive analysis of inflammatory responses, immune regulation, and cell signaling pathways.With a high level of sensitivity and specificity, this array provides accurate and reliable data for understanding the role of cytokines in various disease states, such as cancer, autoimmune disorders, and infectious diseases.
The assay is easy to use, requiring minimal sample input and providing results in a timely manner.Whether you are investigating the impact of cytokines on immune responses, inflammation, or cell proliferation, the Dolphin Cytokine Array 10 Targets is a valuable tool for advancing your research. Trust in the quality and performance of this array to uncover valuable insights into cytokine function and regulation.
| Product Sku: | SARB0012 |
| Size: | 2, 4, or 8 |
| Species Detected: | Dolphin |
| Number of Targets Detected: | 10 |
| Gene Symbols: | CXCL8, IFNG, IL13, IL1RA, IL1RN, IL2, IL6, IL8, TNF, TNFA|TNFSF2 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3)
| IL-2
| IL-6
|
IL-8 (CXCL8)
| IL-13
| IL-17A
| IP-10 (CXCL10)
| TNF alpha
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Dolphin Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
