Human T Cell Response Array (20 targets) (SARB0068)

SKU:
SARB0068
€597 - €1,193

Description

Human T Cell Response Array Product Information

Product Sku:SARB0068
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:20
Gene Symbols:IFNG, IL10, IL12A, IL13, IL17A, IL17F, IL18, IL2, IL21, IL22|IL23A, IL27, IL33, IL4, IL5, IL6, IL7, IL9, TGFB1, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human T Cell Response Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

IL-2
  • Gene Symbol: IL2 IL-2
  • Gene ID: 3558
  • Accession #: P60568
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
IL-7
  • Gene Symbol: IL7
  • Gene ID: 3574
  • Accession #: P13232
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-12 p70
  • Gene Symbol: IL12A NKSF1
  • Gene ID: 3592
  • Accession #: P29459
IL-13
  • Gene Symbol: IL13
  • Gene ID: 3596
  • Accession #: P35225
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
TGF beta 1
  • Gene Symbol: TGFB1 TGFB
  • Gene ID: 7040
  • Accession #: P01137
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 7124
  • Accession #: P01375
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-9
  • Gene Symbol: IL9
  • Gene ID: 3578
  • Accession #: P15248
  

Human T Cell Response Array Kit Components

  • Human T Cell Response Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human T Cell Response Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
IL-2
Target:
IL-4
Target:
IL-5
Target:
IL-6
Target:
IL-7
Target:
IL-10
Target:
IL-12 p70
Target:
IL-13
Target:
IFN-gamma
Target:
TGF beta 1
Target:
TNF alpha
Target:
IL-17A
Target:
IL-9
Target:
IL-22
Target:
IL-17F
Target:
IL-18
Target:
IL-23 p19
Target:
IL-21
Target:
IL-27
Target:
IL-33 (IL-1 F11)
Reactivity:
Human
Application:
Multiplex Array
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