Description
Human Immune Checkpoint Array (11 targets) (SARB0048)
The Human Immune Checkpoint Array (SARB0048) is a comprehensive tool for studying immune checkpoint proteins involved in immune regulation. This array includes 11 targets, providing researchers with a detailed analysis of key molecules that govern immune responses.These targets play crucial roles in modulating immune activity, including inhibiting or activating immune responses to maintain homeostasis. By detecting and analyzing these proteins, researchers can gain insights into immune system function and dysfunction, particularly in conditions such as cancer, autoimmune disorders, and chronic inflammation.
The array is designed for use with human samples and is validated for its sensitivity and specificity in detecting immune checkpoint proteins. By utilizing this array, researchers can accelerate their studies in immunology and cancer research, ultimately aiding in the development of novel therapeutic strategies to manipulate immune checkpoints for clinical benefit.
| Product Sku: | SARB0048 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 11 |
| Gene Symbols: | CD200R1, CD27, CD28, CD40LG, CTLA4, HAVCR1, HAVCR2, ICOS, LAG3, PDCD1|TNFRSF4 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
CD200 R1
| CD27 (TNFRSF7)
| CD28
| CD40 Ligand (TNFSF5)
| CTLA-4 (CD152)
|
ICOS
| LAG-3
| OX40 (TNFRSF4)
| PD-1
| TIM-1 (KIM-1)
|
TIM-3 (KIM-3)
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Immune Checkpoint Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
