Human Immune Checkpoint Array (23 targets) (SARB0047)

SKU:
SARB0047
€485 - €946

Description

Human Immune Checkpoint Array Product Information

Product Sku:SARB0047
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:23
Gene Symbols:CD200, CD200R1, CD27, CD274, CD276, CD28, CD40LG, CD70, CD80, CD86|CTLA4, HAVCR1, HAVCR2, ICOS, ICOSLG, LAG3, PDCD1, PDCD1LG2, TNFRSF14, TNFRSF4, TNFRSF9, TNFSF4, VTCN1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Immune Checkpoint Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

4-1BB (TNFRSF9/CD137)
  • Gene Symbol: TNFRSF9 CD137 ILA
  • Gene ID: 3604
  • Accession #: Q07011
B7-H2 (ICOS Ligand)
  • Gene Symbol: ICOSLG B7H2 B7RP1 ICOSL KIAA0653
  • Gene ID: 102723996; 23308
  • Accession #: O75144
B7-H3 (CD276)
  • Gene Symbol: CD276 B7H3 PSEC0249 UNQ309/PRO352
  • Gene ID: 80381
  • Accession #: Q5ZPR3
B7-H4
  • Gene Symbol: VTCN1 B7H4 UNQ659/PRO1291
  • Gene ID: 79679
  • Accession #: Q7Z7D3
CD200
  • Gene Symbol: CD200 MOX1 MOX2 My033
  • Gene ID: 4345
  • Accession #: P41217
CD200 R1
  • Gene Symbol: CD200R1 CD200R CRTR2 MOX2R OX2R UNQ2522/PRO6015
  • Gene ID: 131450
  • Accession #: Q8TD46
CD27 (TNFRSF7)
  • Gene Symbol: CD27 TNFRSF7
  • Gene ID: 939
  • Accession #: P26842
HVEM (TNFRSF14/CD270)
  • Gene Symbol: TNFRSF14 HVEA HVEM UNQ329/PRO509
  • Gene ID: 8764
  • Accession #: Q92956
CD27 Ligand (TNFSF7)
  • Gene Symbol: CD70 CD27L CD27LG TNFSF7
  • Gene ID: 970
  • Accession #: P32970
CD28
  • Gene Symbol: CD28
  • Gene ID: 940
  • Accession #: P10747
CD40 Ligand (TNFSF5)
  • Gene Symbol: CD40LG CD40L TNFSF5 TRAP
  • Gene ID: 959
  • Accession #: P29965
CD80 (B7-1)
  • Gene Symbol: CD80 CD28LG CD28LG1 LAB7
  • Gene ID: 941
  • Accession #: P33681
CD86 (B7-2)
  • Gene Symbol: CD86 CD28LG2
  • Gene ID: 942
  • Accession #: P42081
  

Human Immune Checkpoint Array Kit Components

  • Human Immune Checkpoint Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Immune Checkpoint Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
4-1BB (TNFRSF9/CD137)
Target:
B7-H2 (ICOS Ligand)
Target:
B7-H3 (CD276)
Target:
B7-H4
Target:
CD200
Target:
CD200 R1
Target:
CD27 (TNFRSF7)
Target:
HVEM (TNFRSF14/CD270)
Target:
CD27 Ligand (TNFSF7)
Target:
CD28
Target:
CD40 Ligand (TNFSF5)
Target:
CD80 (B7-1)
Target:
CD86 (B7-2)
Target:
CTLA-4 (CD152)
Target:
ICOS
Target:
LAG-3
Target:
OX40 (TNFRSF4)
Target:
OX40 Ligand (TNFSF4)
Target:
PD-1
Target:
B7-H1 (CD274/PD-L1)
Target:
PD-L2 (B7-DC)
Target:
TIM-1 (KIM-1)
Target:
TIM-3 (KIM-3)
Reactivity:
Human
Application:
Multiplex Array
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