Description
Human Immune Checkpoint Array (12 targets) (SARB0049)
The Human Immune Checkpoint Array - 12 Targets (SARB0049) is a comprehensive tool for studying immune checkpoint molecules in human samples. This array includes 12 key targets involved in immune regulation, allowing researchers to simultaneously analyze multiple proteins in a single experiment. Each target on the array is carefully selected based on its role in modulating immune responses, making it ideal for investigating pathways involved in immune tolerance, autoimmunity, and anti-tumor immunity. The array provides a high level of sensitivity and specificity, enabling precise quantification of protein expression levels in different biological samples.With this Human Immune Checkpoint Array, researchers can gain valuable insights into the complex interactions between immune cells and their microenvironment.
By analyzing the expression patterns of immune checkpoint molecules, researchers can identify potential biomarkers for disease diagnosis and prognosis, as well as potential targets for therapeutic intervention.Overall, the Human Immune Checkpoint Array - 12 Targets (SARB0049) offers a powerful tool for advancing research in immunology, oncology, and drug development. Its comprehensive design and reliable performance make it a valuable resource for scientists studying immune checkpoint pathways and their implications in various disease states.
| Product Sku: | SARB0049 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 12 |
| Gene Symbols: | CD200, CD274, CD276, CD70, CD80, CD86, ICOSLG, PDCD1LG2, TNFRSF14, TNFRSF9|TNFSF4, VTCN1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
4-1BB (TNFRSF9/CD137)
| B7-H2 (ICOS Ligand)
| B7-H3 (CD276)
| B7-H4
| CD200
|
HVEM (TNFRSF14/CD270)
| CD27 Ligand (TNFSF7)
| CD80 (B7-1)
| CD86 (B7-2)
| OX40 Ligand (TNFSF4)
|
B7-H1 (CD274/PD-L1)
| PD-L2 (B7-DC)
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Immune Checkpoint C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
