Mouse Immune Checkpoint Array (11 targets) (SARB0089)

SKU:
SARB0089
€455 - €880

Description

Mouse Immune Checkpoint Array Product Information

Product Sku:SARB0089
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:11
Gene Symbols:CD27, CD40, CD40LG, CD80, CTLA4, FAS, FASLG, LGALS1, LGALS3, TNFRSF9|TNFSF4
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Immune Checkpoint Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

4-1BB (TNFRSF9/CD137)
  • Gene Symbol: Tnfrsf9 Cd137 Ila Ly63
  • Gene ID: 21942
  • Accession #: P20334
CD27 (TNFRSF7)
  • Gene Symbol: Cd27 Tnfrsf7
  • Gene ID: 21940
  • Accession #: P41272
CD40 (TNFRSF5)
  • Gene Symbol: Cd40 Tnfrsf5
  • Gene ID: 21939
  • Accession #: P27512
CD40 Ligand (TNFSF5)
  • Gene Symbol: Cd40lg Cd40l Tnfsf5
  • Gene ID: 21947
  • Accession #: P27548
CD80 (B7-1)
  • Gene Symbol: Cd80 B7
  • Gene ID: 12519
  • Accession #: Q00609
CTLA-4 (CD152)
  • Gene Symbol: Ctla4 Cd152
  • Gene ID: 12477
  • Accession #: P09793
Fas (TNFRSF6/Apo-1)
  • Gene Symbol: Fas Apt1 Tnfrsf6
  • Gene ID: 14102
  • Accession #: P25446
Fas Ligand (TNFSF6)
  • Gene Symbol: Faslg Apt1lg1 Cd95l Fasl gld Tnfsf6
  • Gene ID: 14103
  • Accession #: P41047
Galectin-1
  • Gene Symbol: Lgals1 Gbp
  • Gene ID: 16852
  • Accession #: P16045
Galectin-3
  • Gene Symbol: Lgals3 mCG_6908
  • Gene ID: 16854
  • Accession #: Q8C253
OX40 Ligand (TNFSF4)
  • Gene Symbol: Tnfsf4 Ox40l Txgp1l
  • Gene ID: 22164
  • Accession #: P43488
    

Mouse Immune Checkpoint Array Kit Components

  • Mouse Immune Checkpoint C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Immune Checkpoint Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
4-1BB (TNFRSF9/CD137)
Target:
CD27 (TNFRSF7)
Target:
CD40 (TNFRSF5)
Target:
CD40 Ligand (TNFSF5)
Target:
CD80 (B7-1)
Target:
CTLA-4 (CD152)
Target:
Fas (TNFRSF6/Apo-1)
Target:
Fas Ligand (TNFSF6)
Target:
Galectin-1
Target:
Galectin-3
Target:
OX40 Ligand (TNFSF4)
Reactivity:
Mouse
Application:
Multiplex Array
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