Description
Human Angiogenesis Array (23 targets) (SARB0020)
The Human Angiogenesis Array (SARB0020) offered by Assay Genie is a powerful tool for studying angiogenesis, the process by which new blood vessels form from existing ones. This array contains 23 targets specifically related to angiogenesis, providing researchers with a comprehensive view of the key factors involved in this vital biological process.With the ability to simultaneously detect multiple proteins in a single sample, this array saves time and resources while providing valuable insights into the molecular mechanisms of angiogenesis. It is suitable for use with a variety of sample types, including serum, plasma, and cell lysates, making it versatile for a wide range of research applications.
By studying the proteins included in the Human Angiogenesis Array, researchers can uncover important insights into angiogenesis-related diseases such as cancer, cardiovascular disease, and diabetic retinopathy. This array is a valuable resource for scientists looking to further our understanding of angiogenesis and develop new therapies for diseases characterized by abnormal blood vessel growth.
| Product Sku: | SARB0020 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 23 |
| Gene Symbols: | ANGPT1, ANGPT2, CCL1, CCL13, CCL7, COL18A1, CSF2, CSF3, CXCL11, FLT4|IL10, IL1A, IL1B, IL2, IL4, KDR, MMP1, MMP9, PECAM1, PLAUR, PLG, TEK, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiopoietin-1
| Angiopoietin-2
| Angiostatin
| Endostatin
| GCSF
|
GM-CSF
| I-309 (TCA-3/CCL1)
| IL-10
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-2
| IL-4
| I-TAC (CXCL11)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
|
MMP-1
| MMP-9
| PECAM-1 (CD31)
| Tie-2
| TNF alpha
|
uPAR
| VEGFR2
| VEGFR3
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Angiogenesis Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
