Human Cytokine Array (60 targets) (SARB0042)

SKU:
SARB0042
€644 - €1,297

Description

Human Cytokine Array Product Information

Product Sku:SARB0042
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:60
Gene Symbols:ADIPOQ, AGRP, ANGPT2, AREG, AXL, BTC, CCL16, CCL19, CCL25, CCL27|CCL28, CCL3, CCL4, CSF3, CXCL1, CXCL11, CXCL2, CXCL3, CXCL5, CXCL8, EGFR, FAS, FGF2, FGF4, FGF9, FIGF, HGF, ICAM1, ICAM3, IGF1R, IGFBP3, IGFBP6, IL11, IL12A, IL12B, IL17A, IL1R1, IL1RL1, IL2RA, IL6R, IL6ST, MIF, MST1, NGF, NTF4, OSM, PGF, PLAUR, THPO, TIMP1, TIMP2, TNFRSF10C, TNFRSF10D, TNFRSF11B, TNFRSF18, TNFRSF1A, TNFRSF1B, TNFSF18, TPO, TYRO3, VEGFA, XCL1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Adiponectin (ACRP30)AgRPAngiopoietin-2AmphiregulinAxl
bFGFbeta-NGFBetacellulin (BTC)CCL28 (MEC)CTACK (CCL27)
DtkEGFRENA-78 (CXCL5)Fas (TNFRSF6/Apo-1)FGF-4
FGF-9GCSFGITR Ligand (TNFSF18)GITR (TNFRSF18)GRO alpha/beta/gamma
GRO alpha (CXCL1)HCC-4 (CCL16)HGFICAM-1 (CD54)ICAM-3 (CD50)
IGFBP-3IGFBP-6IGF-1 RIL-1 R4 (ST2)IL-1 R1
IL-11IL-12 p40IL-12 p70IL-17AIL-2 R alpha
IL-6 RIL-8 (CXCL8)I-TAC (CXCL11)Lymphotactin (XCL1)MIF
MIP-1 alpha (CCL3)MIP-1 beta (CCL4)MIP-3 beta (CCL19)MSP alpha/betaNT-4
Osteoprotegerin (TNFRSF11B)Oncostatin MPLGFgp130TNF RII (TNFRSF1B)
TNF RI (TNFRSF1A)TECK (CCL25)TIMP-1TIMP-2Thrombopoietin (TPO)
TRAIL R3 (TNFRSF10C)TRAIL R4 (TNFRSF10D)uPARVEGF-AVEGF-D

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C7 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Adiponectin (ACRP30)
Target:
AgRP
Target:
Angiopoietin-2
Target:
Amphiregulin
Target:
Axl
Target:
bFGF
Target:
beta-NGF
Target:
Betacellulin (BTC)
Target:
CCL28 (MEC)
Target:
CTACK (CCL27)
Target:
Dtk
Target:
EGFR
Target:
ENA-78 (CXCL5)
Target:
Fas (TNFRSF6/Apo-1)
Target:
FGF-4
Target:
FGF-9
Target:
GCSF
Target:
GITR Ligand (TNFSF18)
Target:
GITR (TNFRSF18)
Target:
GRO alpha/beta/gamma
Target:
GRO alpha (CXCL1)
Target:
HCC-4 (CCL16)
Target:
HGF
Target:
ICAM-1 (CD54)
Target:
ICAM-3 (CD50)
Target:
IGFBP-3
Target:
IGFBP-6
Target:
IGF-1 R
Target:
IL-1 R4 (ST2)
Target:
IL-1 R1
Target:
IL-11
Target:
IL-12 p40
Target:
IL-12 p70
Target:
IL-17A
Target:
IL-2 R alpha
Target:
IL-6 R
Target:
IL-8 (CXCL8)
Target:
I-TAC (CXCL11)
Target:
Lymphotactin (XCL1)
Target:
MIF
Target:
MIP-1 alpha (CCL3)
Target:
MIP-1 beta (CCL4)
Target:
MIP-3 beta (CCL19)
Target:
MSP alpha/beta
Reactivity:
Human
Application:
Multiplex Array
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