Rat Cytokine Array (19 targets) (SARB0101)

SKU:
SARB0101
€510 - €989

Description

Rat Cytokine Array Product Information

Product Sku:SARB0101
Size:2, 4, or 8
Species Detected:Rat
Number of Targets Detected:19
Gene Symbols:CCL2, CCL20, CNTF, CSF2, CX3CL1, CXCL2, CXCL3, CXCL5, IFNG, IL10, IL1A, IL1B, IL4, IL6, LEP, NGF, TIMP1, TNF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Rat Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

CINC-2
  • Gene Symbol: CXCL3
  • Accession #: Q10746
CINC-3
  • Gene Symbol: Cxcl2
  • Accession #: P30348
CNTF
  • Gene Symbol: CNTF
  • Accession #: P20294
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1
  • Accession #: Q6IRF7
GM-CSF
  • Gene Symbol: CSF2
  • Accession #: P48750
IFN-gamma
  • Gene Symbol: IFNG
  • Accession #: P01581
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Accession #: P16598
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Accession #: Q63264
IL-4
  • Gene Symbol: IL4
  • Accession #: P20096
IL-6
  • Gene Symbol: IL6
  • Accession #: P20607
IL-10
  • Gene Symbol: IL10
  • Accession #: P29456
LIX
  • Gene Symbol: Cxcl5
  • Accession #: P97885
Leptin
  • Gene Symbol: LEP
  • Accession #: P50596
  

Rat Cytokine Array Kit Components

  • Rat Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Rat Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
CINC-2
Target:
CINC-3
Target:
CNTF
Target:
Fractalkine (CX3CL1)
Target:
GM-CSF
Target:
IFN-gamma
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-4
Target:
IL-6
Target:
IL-10
Target:
LIX
Target:
Leptin
Target:
MCP-1 (CCL2)
Target:
MIP-3 alpha (CCL20)
Target:
beta-NGF
Target:
TIMP-1
Target:
TNF alpha
Target:
VEGF-A
Reactivity:
Rat
Application:
Multiplex Array
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