Rat Cytokine Array (19 targets) (SARB0101)
- SKU:
- SARB0101
- Product Type:
- Protein Array
- Analytes:
- CINC-2
- CINC-3
- CNTF
- Fractalkine (CX3CL1)
- GM-CSF
- IFN-gamma
- IL-1 alpha (IL-1 F1)
- IL-1 beta (IL-1 F2)
- IL-4
- IL-6
- IL-10
- LIX
- Leptin
- MCP-1 (CCL2)
- MIP-3 alpha (CCL20)
- beta-NGF
- TIMP-1
- TNF alpha
- VEGF-A
- Reactivity:
- Rat
- Applications:
- Multiplex Array
Description
Rat Cytokine Array (19 targets) (SARB0101)
The Rat Cytokine Array - 19 Targets (SARB0101) is a comprehensive research tool designed for the simultaneous detection and quantification of 19 different cytokines in rat samples. This array allows researchers to analyze the expression levels of key cytokines involved in immune response, inflammation, and other biological processes.With high sensitivity and specificity, the Rat Cytokine Array - 19 Targets provides reliable and accurate results, making it ideal for various research applications. The array is easy to use and requires minimal sample input, making it a cost-effective solution for studying cytokine expression profiles in rat models of disease.
Whether studying inflammatory pathways, immune responses, or the effects of therapeutic interventions, the Rat Cytokine Array - 19 Targets provides valuable data that can help further our understanding of cytokine signaling in health and disease. Researchers in immunology, oncology, and other fields will benefit from the comprehensive information this array can provide, leading to new insights and potential therapeutic targets.
| Product Sku: | SARB0101 |
| Size: | 2, 4, or 8 |
| Species Detected: | Rat |
| Number of Targets Detected: | 19 |
| Gene Symbols: | CCL2, CCL20, CNTF, CSF2, CX3CL1, CXCL2, CXCL3, CXCL5, IFNG, IL10, IL1A, IL1B, IL4, IL6, LEP, NGF, TIMP1, TNF, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
CINC-2
| CINC-3
| CNTF
| Fractalkine (CX3CL1)
| GM-CSF
|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-4
| IL-6
|
IL-10
| LIX
| Leptin
| MCP-1 (CCL2)
| MIP-3 alpha (CCL20)
|
beta-NGF
| TIMP-1
| TNF alpha
| VEGF-A
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Rat Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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