Porcine Cytokine Array (48 targets) (SARB0095)

SKU:
SARB0095
€738 - €1,485

Description

Porcine Cytokine Array Product Information

Product Sku:SARB0095
Size:2, 4, or 8
Species Detected:Porcine
Number of Targets Detected:48
Gene Symbols:ANGPT1, CCL4, CCL5, CSF2, CXCL8, CXCL9, DCN, EPO, FGF21, IFN-BETA|IFNB, IFNB1, IFNG, IGF2, IGFBP5, IGIF, IL10, IL12A, IL13, IL15, IL17, IL17A, IL17F, IL18, IL1A, IL1B, IL1RN, IL21, IL22, IL28B, IL4, IL6, IL8, INS, INTERFERON-BETA-1, INTERFERON-BETA/POIFN-BETA, IRAP1, KITLG, MGF, MIF, MIP1B, NCAM1, PDGFB, PECAM1, PLGF, SCY4A, SCYA5, TGFB1, TIMP-2, TIMP1, TIMP2, TNF, TNFA, TNFRSF11B, TNFRSF12A, TNFSF2, VEGF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Porcine Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Angiopoietin-1CCL3L1DecorinEotaxin-1 (CCL11)Erythropoietin
FGF-21Galectin-9GASP-1 (WFIKKNRP)GM-CSFIFN alpha
IFN-betaIFN-gammaIGF-2IGFBP-5IL-1 alpha (IL-1 F1)
IL-1 beta (IL-1 F2)IL-1 Ra (IL-1 F3)IL-10IL-12 p70IL-13
IL-15IL-17AIL-17FIL-18IL-21
IL-22IL-28BIL-4IL-6399500
IL-8 (CXCL8)396880InsulinMIFMIG (CXCL9)
MIP-1 beta (CCL4)NCAM-1 (CD56)Osteoprotegerin (TNFRSF11B)PDGF-BBPECAM-1 (CD31)
PLGF-2RANTES (CCL5)SCFTGF alphaTGF beta 1
TIMP-1TIMP-2TNF alphaTWEAK R (TNFRSF12)VEGF-A

Porcine Cytokine Array Kit Components

  • Porcine Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Porcine Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Angiopoietin-1
Target:
CCL3L1
Target:
Decorin
Target:
Eotaxin-1 (CCL11)
Target:
Erythropoietin
Target:
FGF-21
Target:
Galectin-9
Target:
GASP-1 (WFIKKNRP)
Target:
GM-CSF
Target:
IFN alpha
Target:
IFN-beta
Target:
IFN-gamma
Target:
IGF-2
Target:
IGFBP-5
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-1 Ra (IL-1 F3)
Target:
IL-10
Target:
IL-12 p70
Target:
IL-13
Target:
IL-15
Target:
IL-17A
Target:
IL-17F
Target:
IL-18
Target:
IL-21
Target:
IL-22
Target:
IL-28B
Target:
IL-4
Target:
IL-6
Target:
IL-8 (CXCL8)
Target:
Insulin
Target:
MIF
Target:
MIG (CXCL9)
Target:
MIP-1 beta (CCL4)
Target:
NCAM-1 (CD56)
Target:
Osteoprotegerin (TNFRSF11B)
Target:
PDGF-BB
Target:
PECAM-1 (CD31)
Target:
PLGF-2
Target:
RANTES (CCL5)
Target:
SCF
Reactivity:
Porcine
Application:
Multiplex Array
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