Description
Porcine Chemokine Array (12 targets) (SARB0094)
The Porcine Chemokine Array - 12 targets (SARB0094) from Assay Genie is a powerful tool for studying the expression levels of 12 different chemokines in porcine samples. This array allows researchers to simultaneously detect and quantify the levels of various chemokines, such as MCP-1, MIP-1β, and RANTES, in a single experiment.With high sensitivity and specificity, this array provides valuable insights into the chemokine profiles of porcine samples, enabling researchers to investigate inflammatory responses, immune cell recruitment, and other processes regulated by chemokines.
The array is easy to use, requiring minimal sample input, and provides reliable and reproducible results.Whether studying porcine immune responses, inflammation, or other biological processes, the Porcine Chemokine Array - 12 targets (SARB0094) is a versatile and efficient tool for researchers looking to profile multiple chemokines in their samples. Trust Assay Genie for high-quality arrays that deliver accurate and meaningful data for your research needs.
| Product Sku: | SARB0094 |
| Size: | 2, 4, or 8 |
| Species Detected: | Porcine |
| Number of Targets Detected: | 12 |
| Gene Symbols: | CCL4, CCL5, CXCL9, IFN-BETA, IFNB, IFNB1, IFNG, IL1A, IL1B, INTERFERON-BETA-1|INTERFERON-BETA/POIFN-BETA, MIF, MIP1B, SCY4A, SCYA5, TNF, TNFA, TNFSF2 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
CCL3L1
| Eotaxin-1 (CCL11)
| IFN alpha
| IFN-beta
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| MIF
| MIG (CXCL9)
| MIP-1 beta (CCL4)
|
RANTES (CCL5)
| TNF alpha
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Porcine Chemokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
