Description
Mouse Chemokine Array (25 targets) (SARB0076)
The Mouse Chemokine Array (SARb0076) is a powerful tool for researchers studying immune responses and inflammation in mice. This array contains antibodies for detecting 25 different mouse chemokines, including key players like MCP-1, MIP-1 alpha, and RANTES.With this array, researchers can simultaneously analyze multiple chemokines in a single experiment, providing a comprehensive view of the immune signaling pathways involved in various physiological and pathological processes. The array is suitable for use with various sample types, including cell lysates, tissue homogenates, and serum/plasma samples.The Mouse Chemokine Array is easy to use, requiring minimal sample volume and providing rapid results.
It is a valuable resource for studies in immunology, inflammation, and cancer research, offering insights into the complex network of chemokine-mediated immune responses in mouse models.Overall, the Mouse Chemokine Array (SARb0076) is a reliable and efficient tool for investigating the role of chemokines in immune regulation and disease pathogenesis in mouse models, making it a valuable asset for researchers seeking to advance our understanding of immune function and develop novel therapeutic strategies.
| Product Sku: | SARB0076 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 25 |
| Gene Symbols: | CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL21, CCL22, CCL24|CCL25, CCL27, CCL3, CCL5, CCL9, CX3CL1, CXCL1, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL5, CXCL9, PF4 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
6Ckine (CCL21)
| BLC (CXCL13)
| CTACK (CCL27)
| CXCL16
| Eotaxin-1 (CCL11)
|
Eotaxin-2 (MPIF-2/CCL24)
| Fractalkine (CX3CL1)
| I-TAC (CXCL11)
| KC (CXCL1)
| LIX
|
MCP-1 (CCL2)
| MCP-5
| MDC (CCL22)
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
MIP-1 gamma
| MIP-2
| MIP-3 beta (CCL19)
| MIP-3 alpha (CCL20)
| Platelet Factor 4 (CXCL4)
|
RANTES (CCL5)
| SDF-1 (CXCL12)
| TARC (CCL17)
| I-309 (TCA-3/CCL1)
| TECK (CCL25)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Chemokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
