Mouse Chemokine Array (25 targets) (SARB0076)

SKU:
SARB0076
€631 - €1,267

Description

Mouse Chemokine Array Product Information

Product Sku:SARB0076
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:25
Gene Symbols:CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL21, CCL22, CCL24|CCL25, CCL27, CCL3, CCL5, CCL9, CX3CL1, CXCL1, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL5, CXCL9, PF4
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Chemokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

6Ckine (CCL21)
  • Gene Symbol: CCL21
  • Accession #: P86792
BLC (CXCL13)
  • Gene Symbol: CXCL13
  • Accession #: O55038
CTACK (CCL27)
  • Gene Symbol: CCL27
  • Accession #: Q9Z1X0
CXCL16
  • Gene Symbol: CXCL16
  • Accession #: Q5F2D5
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Accession #: P48298
Eotaxin-2 (MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Accession #: Q9JKC0
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1
  • Accession #: O35188
I-TAC (CXCL11)
  • Gene Symbol: CXCL11
  • Accession #: Q9JHH5
KC (CXCL1)
  • Gene Symbol: CXCL1
  • Accession #: P12850
LIX
  • Gene Symbol: Cxcl5
  • Accession #: P50228
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Accession #: Q9QYD7
MCP-5
  • Gene Symbol: Ccl12
  • Accession #: Q9QYD6
MDC (CCL22)
  • Gene Symbol: CCL22
  • Accession #: O88430
  

Mouse Chemokine Array Kit Components

  • Mouse Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Chemokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
6Ckine (CCL21)
Target:
BLC (CXCL13)
Target:
CTACK (CCL27)
Target:
CXCL16
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
Fractalkine (CX3CL1)
Target:
I-TAC (CXCL11)
Target:
KC (CXCL1)
Target:
LIX
Target:
MCP-1 (CCL2)
Target:
MCP-5
Target:
MDC (CCL22)
Target:
MIG (CXCL9)
Target:
MIP-1 alpha (CCL3)
Target:
MIP-1 gamma
Target:
MIP-2
Target:
MIP-3 beta (CCL19)
Target:
MIP-3 alpha (CCL20)
Target:
Platelet Factor 4 (CXCL4)
Target:
RANTES (CCL5)
Target:
SDF-1 (CXCL12)
Target:
TARC (CCL17)
Target:
I-309 (TCA-3/CCL1)
Target:
TECK (CCL25)
Reactivity:
Mouse
Application:
Multiplex Array
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