Human Chemokine Array (38 targets) (SARB0030)

SKU:
SARB0030
€543 - €1,075

Description

Human Chemokine Array Product Information

Product Sku:SARB0030
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:38
Gene Symbols:CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20|CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CXCL9, PPBP, XCL1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Chemokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

BLC (CXCL13)CCL28 (MEC)Ck beta 8-1 (CCL23)CTACK (CCL27)CXCL16
ENA-78 (CXCL5)Eotaxin-1 (CCL11)Eotaxin-2 (MPIF-2/CCL24)Eotaxin-3 (CCL26)Fractalkine (CX3CL1)
GCP-2 (CXCL6)GRO alpha/beta/gammaGRO alpha (CXCL1)HCC-4 (CCL16)I-309 (TCA-3/CCL1)
I-TAC (CXCL11)IL-8 (CXCL8)IP-10 (CXCL10)Lymphotactin (XCL1)MCP-1 (CCL2)
MCP-2 (CCL8)MCP-3 (MARC/CCL7)MCP-4 (CCL13)MDC (CCL22)MIG (CXCL9)
MIP-1 alpha (CCL3)MIP-1 beta (CCL4)MIP-1 delta (CCL15)MIP-3 alpha (CCL20)MIP-3 beta (CCL19)
MPIF-1 (CCL23)NAP-2 (PPBP/CXCL7)PARC (CCL18)RANTES (CCL5)SDF-1 alpha (CXCL12 alpha)
SDF-1 beta (CXCL12 beta)TARC (CCL17)TECK (CCL25)  

Human Chemokine Array Kit Components

  • Human Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Chemokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
BLC (CXCL13)
Target:
CCL28 (MEC)
Target:
Ck beta 8-1 (CCL23)
Target:
CTACK (CCL27)
Target:
CXCL16
Target:
ENA-78 (CXCL5)
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
Eotaxin-3 (CCL26)
Target:
Fractalkine (CX3CL1)
Target:
GCP-2 (CXCL6)
Target:
GRO alpha/beta/gamma
Target:
GRO alpha (CXCL1)
Target:
HCC-4 (CCL16)
Target:
I-309 (TCA-3/CCL1)
Target:
I-TAC (CXCL11)
Target:
IL-8 (CXCL8)
Target:
IP-10 (CXCL10)
Target:
Lymphotactin (XCL1)
Target:
MCP-1 (CCL2)
Target:
MCP-2 (CCL8)
Target:
MCP-3 (MARC/CCL7)
Target:
MCP-4 (CCL13)
Target:
MDC (CCL22)
Target:
MIG (CXCL9)
Target:
MIP-1 alpha (CCL3)
Target:
MIP-1 beta (CCL4)
Target:
MIP-1 delta (CCL15)
Target:
MIP-3 alpha (CCL20)
Target:
MIP-3 beta (CCL19)
Target:
MPIF-1 (CCL23)
Target:
NAP-2 (PPBP/CXCL7)
Target:
PARC (CCL18)
Target:
RANTES (CCL5)
Target:
SDF-1 alpha (CXCL12 alpha)
Target:
SDF-1 beta (CXCL12 beta)
Target:
TARC (CCL17)
Target:
TECK (CCL25)
Reactivity:
Human
Application:
Multiplex Array
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