Rat Chemokine Array (15 targets) (SARB0100)

SKU:
SARB0100
€548 - €1,073

Description

Rat Chemokine Array Product Information

Product Sku:SARB0100
Size:2, 4, or 8
Species Detected:Rat
Number of Targets Detected:15
Gene Symbols:CCL11, CCL2, CCL20, CCL27, CCL3, CCL4, CX3CL1, CXCL1, CXCL2, CXCL3|CXCL5, CXCL9, IL1A, PPBP, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Rat Chemokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

MIP-3 alpha (CCL20)
  • Gene Symbol: Ccl20 Scya20 St38
  • Gene ID: 29538
  • Accession #: P97884
CINC-1 (CXCL1)
  • Gene Symbol: Cxcl1 Cinc1 Gro Scyb1
  • Gene ID: 81503
  • Accession #: P14095
CINC-2
  • Gene Symbol: Cxcl3 Cinc2
  • Gene ID: 171551
  • Accession #: Q10746
CINC-3
  • Gene Symbol: Cxcl2 Cinc3 Mip-2 Mip2 Scyb2
  • Gene ID: 114105
  • Accession #: P30348
CTACK (CCL27)
  • Gene Symbol: Ccl27 Ccl27_predicted rCG_54915
  • Gene ID: 362505
  • Accession #: D4AAL6
Eotaxin-1 (CCL11)
  • Gene Symbol: Ccl11 Scya11
  • Gene ID: 29397
  • Accession #: P97545
Fractalkine (CX3CL1)
  • Gene Symbol: Cx3cl1 rCG_39059
  • Accession #: Q6IRF7
IL-1 alpha (IL-1 F1)
  • Gene Symbol: Il1a
  • Gene ID: 24493
  • Accession #: P16598
LIX
  • Gene Symbol: Cxcl5 Scyb5
  • Gene ID: 60665
  • Accession #: P97885
MCP-1 (CCL2)
  • Gene Symbol: Ccl2 Je Mcp1 Scya2
  • Gene ID: 24770
  • Accession #: P14844
MIG (CXCL9)
  • Gene Symbol: Cxcl9 Mig rCG_60435
  • Gene ID: 246759
  • Accession #: Q8K4B1
MIP-1 beta (CCL4)
  • Gene Symbol: Ccl4 Mip1b Scya4
  • Gene ID: 116637
  • Accession #: P50230
MIP-1 alpha (CCL3)
  • Gene Symbol: Ccl3 Mip1a Scya3
  • Gene ID: 25542
  • Accession #: P50229
  

Rat Chemokine Array Kit Components

  • Rat Chemokine Array 1
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Rat Chemokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
MIP-3 alpha (CCL20)
Target:
CINC-1 (CXCL1)
Target:
CINC-2
Target:
CINC-3
Target:
CTACK (CCL27)
Target:
Eotaxin-1 (CCL11)
Target:
Fractalkine (CX3CL1)
Target:
IL-1 alpha (IL-1 F1)
Target:
LIX
Target:
MCP-1 (CCL2)
Target:
MIG (CXCL9)
Target:
MIP-1 beta (CCL4)
Target:
MIP-1 alpha (CCL3)
Target:
TCK-1 (CXCL7)
Target:
TNF alpha
Reactivity:
Rat
Application:
Multiplex Array
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