Mouse Chemokine Array (25 targets) (SARB0076)
- SKU:
- SARB0076
- Product Type:
- Protein Array
- Analytes:
- 6Ckine (CCL21)
- BLC (CXCL13)
- CTACK (CCL27)
- CXCL16
- Eotaxin-1 (CCL11)
- Eotaxin-2 (MPIF-2/CCL24)
- Fractalkine (CX3CL1)
- I-TAC (CXCL11)
- KC (CXCL1)
- LIX
- MCP-1 (CCL2)
- MCP-5
- MDC (CCL22)
- MIG (CXCL9)
- MIP-1 alpha (CCL3)
- MIP-1 gamma
- MIP-2
- MIP-3 beta (CCL19)
- MIP-3 alpha (CCL20)
- Platelet Factor 4 (CXCL4)
- RANTES (CCL5)
- SDF-1 (CXCL12)
- TARC (CCL17)
- I-309 (TCA-3/CCL1)
- TECK (CCL25)
- Reactivity:
- Mouse
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
Product Sku: | SARB0076 |
Size: | 2, 4, or 8 |
Species Detected: | Mouse |
Number of Targets Detected: | 25 |
Gene Symbols: | CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL21, CCL22, CCL24|CCL25, CCL27, CCL3, CCL5, CCL9, CX3CL1, CXCL1, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL5, CXCL9, PF4 |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
6Ckine (CCL21)
| BLC (CXCL13)
| CTACK (CCL27)
| CXCL16
| Eotaxin-1 (CCL11)
|
Eotaxin-2 (MPIF-2/CCL24)
| Fractalkine (CX3CL1)
| I-TAC (CXCL11)
| KC (CXCL1)
| LIX
|
MCP-1 (CCL2)
| MCP-5
| MDC (CCL22)
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
MIP-1 gamma
| MIP-2
| MIP-3 beta (CCL19)
| MIP-3 alpha (CCL20)
| Platelet Factor 4 (CXCL4)
|
RANTES (CCL5)
| SDF-1 (CXCL12)
| TARC (CCL17)
| I-309 (TCA-3/CCL1)
| TECK (CCL25)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Chemokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Biotinylated Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated Streptavidin |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |