Human Cytokine Array (32 targets) (SARB0035)

SKU:
SARB0035
€843 - €1,297

Description

Human Cytokine Array Product Information

Product Sku:SARB0035
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:32
Gene Symbols:AHSG, ALB, AMICA1, ANGPT4, CD163, CLU, CRTAM, CSF3R, CST3, CXCL14|DLL1, FGF1, FOLR1, GPRASP1, GPRASP2, IL17RB, IL27, LAG3, LDLR, PGA3, PGA4, PGA5, PRAP1, RBP4, SDC1, SOST, SPINT2, TFPI, THBS1, TNFRSF13B, TNFSF11, TNFSF13B, TNNI2, WISP1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

aFGF (FGF-1)
  • Gene Symbol: FGF1
  • Gene ID: 2246
  • Accession #: P05230
Albumin
  • Gene Symbol: ALB
  • Gene ID: 213
  • Accession #: P02768
AMICA
  • Gene Symbol: AMICA1
  • Gene ID: 120425
  • Accession #: Q86YT9
Angiopoietin-4
  • Gene Symbol: ANGPT4
  • Gene ID: 51378
  • Accession #: Q9Y264
BAFF
  • Gene Symbol: TNFSF13B
  • Gene ID: 10673
  • Accession #: Q9Y275
CD163
  • Gene Symbol: CD163
  • Gene ID: 9332
  • Accession #: Q86VB7
Clusterin
  • Gene Symbol: CLU
  • Gene ID: 1191
  • Accession #: P10909
CRTAM
  • Gene Symbol: CRTAM
  • Gene ID: 56253
  • Accession #: O95727
CXCL14 (BRAK)
  • Gene Symbol: CXCL14
  • Gene ID: 9547
  • Accession #: O95715
Cystatin C
  • Gene Symbol: CST3
  • Gene ID: 1471
  • Accession #: P01034
DLL1
  • Gene Symbol: DLL1
  • Gene ID: 28514
  • Accession #: O00548
Fetuin A
  • Gene Symbol: AHSG
  • Gene ID: 197
  • Accession #: P02765
FOLR1
  • Gene Symbol: FOLR1
  • Gene ID: 2348
  • Accession #: P15328
  

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C12 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
aFGF (FGF-1)
Target:
Albumin
Target:
AMICA
Target:
Angiopoietin-4
Target:
BAFF
Target:
CD163
Target:
Clusterin
Target:
CRTAM
Target:
CXCL14 (BRAK)
Target:
Cystatin C
Target:
DLL1
Target:
Fetuin A
Target:
FOLR1
Target:
GASP-1 (WFIKKNRP)
Target:
GASP-2 (WFIKKN)
Target:
GCSF R (CD114)
Target:
HAI-2
Target:
IL-17 RB
Target:
IL-27
Target:
LAG-3
Target:
LDL R
Target:
Pepsinogen 1
Target:
RBP4
Target:
SOST
Target:
Syndecan-1
Target:
TACI (TNFRSF13B)
Target:
TFPI
Target:
Thrombospondin 1
Target:
TRANCE (TNFSF11)
Target:
Troponin I
Target:
uPA
Target:
WISP-1 (CCN4)
Reactivity:
Human
Application:
Multiplex Array
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