Human Cytokine Array (32 targets) (SARB0035)
- SKU:
- SARB0035
- Product Type:
- Protein Array
- Analytes:
- aFGF (FGF-1)
- Albumin
- AMICA
- Angiopoietin-4
- BAFF
- CD163
- Clusterin
- CRTAM
- CXCL14 (BRAK)
- Cystatin C
- DLL1
- Fetuin A
- FOLR1
- GASP-1 (WFIKKNRP)
- GASP-2 (WFIKKN)
- GCSF R (CD114)
- HAI-2
- IL-17 RB
- IL-27
- LAG-3
- LDL R
- Pepsinogen 1
- RBP4
- SOST
- Syndecan-1
- TACI (TNFRSF13B)
- TFPI
- Thrombospondin 1
- TRANCE (TNFSF11)
- Troponin I
- uPA
- WISP-1 (CCN4)
- Reactivity:
- Human
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
| Product Sku: | SARB0035 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 32 |
| Gene Symbols: | AHSG, ALB, AMICA1, ANGPT4, CD163, CLU, CRTAM, CSF3R, CST3, CXCL14|DLL1, FGF1, FOLR1, GPRASP1, GPRASP2, IL17RB, IL27, LAG3, LDLR, PGA3, PGA4, PGA5, PRAP1, RBP4, SDC1, SOST, SPINT2, TFPI, THBS1, TNFRSF13B, TNFSF11, TNFSF13B, TNNI2, WISP1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
aFGF (FGF-1)
| Albumin
| AMICA
| Angiopoietin-4
| BAFF
|
CD163
| Clusterin
| CRTAM
| CXCL14 (BRAK)
| Cystatin C
|
DLL1
| Fetuin A
| FOLR1
| GASP-1 (WFIKKNRP)
| GASP-2 (WFIKKN)
|
GCSF R (CD114)
| HAI-2
| IL-17 RB
| IL-27
| LAG-3
|
LDL R
| Pepsinogen 1
| RBP4
| SOST
| Syndecan-1
|
TACI (TNFRSF13B)
| TFPI
| Thrombospondin 1
| TRANCE (TNFSF11)
| Troponin I
|
uPA
| WISP-1 (CCN4)
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C12 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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