Rat AKI Array (7 targets) (SARB0099)

SKU:
SARB0099
€526 - €1,025

Description

Rat AKI Array Product Information

Product Sku:SARB0099
Size:2, 4, or 8
Species Detected:Rat
Number of Targets Detected:7
Gene Symbols:CCL2, CST3, FABP1, HAVCR1, LCN2, TIMP1, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Rat AKI Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

TIM-1 (KIM-1)
  • Gene Symbol: HAVCR1
  • Accession #: O54947
FABP1
  • Gene Symbol: FABP1
  • Accession #: P00507
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Accession #: P14844
Cystatin C
  • Gene Symbol: CST3
  • Accession #: P14841
Lipocalin-2 (NGAL)
  • Gene Symbol: LCN2
  • Accession #: P30152

Rat AKI Array Kit Components

  • Rat Acute Kidney Injury (AKI) Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Rat AKI Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
TIM-1 (KIM-1)
Target:
FABP1
Target:
MCP-1 (CCL2)
Target:
Cystatin C
Target:
Lipocalin-2 (NGAL)
Target:
TIMP-1
Target:
VEGF-A
Reactivity:
Rat
Application:
Multiplex Array
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