Human AKI Array (20 targets) (SARB0016)

SKU:
SARB0016
€644 - €1,297

Description

Human AKI Array Product Information

Product Sku:SARB0016
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:20
Gene Symbols:ALB, B2M, CALB1, CCL2, CLU, CST3, CXCL10, CXCL16, FABP1, GPNMB|HAVCR1, HGF, LCN2, MIF, SPP1, TFF3, TIMP1, TNFRSF1A, VCAM1, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human AKI Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

TIM-1 (KIM-1)
  • Gene Symbol: HAVCR1
  • Gene ID: 26762
  • Accession #: Q96D42
Albumin
  • Gene Symbol: ALB
  • Gene ID: 213
  • Accession #: P02768
Osteopontin (SPP1)
  • Gene Symbol: SPP1
  • Gene ID: 6696
  • Accession #: P10451
TFF3 (Trefoil Factor 3)
  • Gene Symbol: TFF3
  • Gene ID: 7033
  • Accession #: Q07654
Beta-2 Microglobulin
  • Gene Symbol: B2M
  • Gene ID: 567
  • Accession #: P61769
Clusterin
  • Gene Symbol: CLU
  • Gene ID: 1191
  • Accession #: P10909
CXCL16
  • Gene Symbol: CXCL16
  • Gene ID: 58191
  • Accession #: Q9H2A7
Osteoactivin (GPNMB)
  • Gene Symbol: GPNMB
  • Gene ID: 10457
  • Accession #: Q14956
FABP1
  • Gene Symbol: FABP1
  • Gene ID: 2168
  • Accession #: P07148
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
TNF RI (TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: 7132
  • Accession #: P19438
Calbindin
  • Gene Symbol: CALB1
  • Gene ID: 793
  • Accession #: P05937
IP-10 (CXCL10)
  • Gene Symbol: CXCL10
  • Gene ID: 3627
  • Accession #: P02778
  

Human AKI Array Kit Components

  • Human Acute Kidney Injury (AKI) Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human AKI Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
TIM-1 (KIM-1)
Target:
Albumin
Target:
Osteopontin (SPP1)
Target:
TFF3 (Trefoil Factor 3)
Target:
Beta-2 Microglobulin
Target:
Clusterin
Target:
CXCL16
Target:
Osteoactivin (GPNMB)
Target:
FABP1
Target:
MCP-1 (CCL2)
Target:
TNF RI (TNFRSF1A)
Target:
Calbindin
Target:
IP-10 (CXCL10)
Target:
Cystatin C
Target:
HGF
Target:
MIF
Target:
Lipocalin-2 (NGAL)
Target:
TIMP-1
Target:
VCAM-1 (CD106)
Target:
VEGF-A
Reactivity:
Human
Application:
Multiplex Array
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