Human Autophagy Array (20 targets) (SARB0023)

SKU:
SARB0023
€531 - €1,047

Description

Human Autophagy Array Product Information

Product Sku:SARB0023
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:20
Gene Symbols:ATG10, ATG12, ATG13, ATG3, ATG4A, ATG4B, ATG5, ATG7, BECN1, BNIP3L|DDR2, GABARAP, LAMP1, MAP1LC3A, MAP1LC3B, NBN, RHEB, RPS6KA5, SNCA, SQSTM1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Autophagy Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

ATG12
  • Gene Symbol: ATG12 APG12 APG12L
  • Gene ID: 9140
  • Accession #: O94817
ATG7
  • Gene Symbol: ATG7 APG7L
  • Gene ID: 10533
  • Accession #: O95352
ATG10
  • Gene Symbol: ATG10 APG10L PP12616
  • Gene ID: 83734
  • Accession #: Q9H0Y0
ATG13
  • Gene Symbol: ATG13 KIAA0652
  • Gene ID: 9776
  • Accession #: O75143
ATG3
  • Gene Symbol: ATG3 APG3 APG3L
  • Gene ID: 64422
  • Accession #: Q9NT62
ATG4A
  • Gene Symbol: ATG4A APG4A AUTL2
  • Gene ID: 115201
  • Accession #: Q8WYN0
ATG4B
  • Gene Symbol: ATG4B APG4B AUTL1 KIAA0943
  • Gene ID: 23192
  • Accession #: Q9Y4P1
ATG5
  • Gene Symbol: ATG5 APG5L ASP
  • Gene ID: 9474
  • Accession #: Q9H1Y0
Beclin
  • Gene Symbol: BECN1 GT197
  • Gene ID: 8678
  • Accession #: Q14457
BNIP3L
  • Gene Symbol: BNIP3L BNIP3A BNIP3H NIX
  • Gene ID: 665
  • Accession #: O60238
DDR2
  • Gene Symbol: DDR2 NTRKR3 TKT TYRO10
  • Gene ID: 4921
  • Accession #: Q16832
GABARAP
  • Gene Symbol: GABARAP FLC3B HT004
  • Gene ID: 11337
  • Accession #: O95166
LAMP1 (CD107a)
  • Gene Symbol: LAMP1
  • Gene ID: 3916
  • Accession #: P11279
  

Human Autophagy Array Kit Components

  • Human Autophagy Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Autophagy Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
ATG12
Target:
ATG7
Target:
ATG10
Target:
ATG13
Target:
ATG3
Target:
ATG4A
Target:
ATG4B
Target:
ATG5
Target:
Beclin
Target:
BNIP3L
Target:
DDR2
Target:
GABARAP
Target:
LAMP1 (CD107a)
Target:
LC3a
Target:
LC3B
Target:
NBS1
Target:
P62
Target:
RHEB
Target:
MSK1
Target:
Alpha-synuclein
Reactivity:
Human
Application:
Multiplex Array
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