Human Autophagy Array (20 targets)

Product Sku:	SARB0023
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	20
Gene Symbols:	ATG10, ATG12, ATG13, ATG3, ATG4A, ATG4B, ATG5, ATG7, BECN1, BNIP3L\|DDR2, GABARAP, LAMP1, MAP1LC3A, MAP1LC3B, NBN, RHEB, RPS6KA5, SNCA, SQSTM1
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
ATG12 Gene Symbol: ATG12 APG12 APG12L Gene ID: 9140 Accession #: O94817	ATG7 Gene Symbol: ATG7 APG7L Gene ID: 10533 Accession #: O95352	ATG10 Gene Symbol: ATG10 APG10L PP12616 Gene ID: 83734 Accession #: Q9H0Y0	ATG13 Gene Symbol: ATG13 KIAA0652 Gene ID: 9776 Accession #: O75143	ATG3 Gene Symbol: ATG3 APG3 APG3L Gene ID: 64422 Accession #: Q9NT62
ATG4A Gene Symbol: ATG4A APG4A AUTL2 Gene ID: 115201 Accession #: Q8WYN0	ATG4B Gene Symbol: ATG4B APG4B AUTL1 KIAA0943 Gene ID: 23192 Accession #: Q9Y4P1	ATG5 Gene Symbol: ATG5 APG5L ASP Gene ID: 9474 Accession #: Q9H1Y0	Beclin Gene Symbol: BECN1 GT197 Gene ID: 8678 Accession #: Q14457	BNIP3L Gene Symbol: BNIP3L BNIP3A BNIP3H NIX Gene ID: 665 Accession #: O60238
DDR2 Gene Symbol: DDR2 NTRKR3 TKT TYRO10 Gene ID: 4921 Accession #: Q16832	GABARAP Gene Symbol: GABARAP FLC3B HT004 Gene ID: 11337 Accession #: O95166	LAMP1 (CD107a) Gene Symbol: LAMP1 Gene ID: 3916 Accession #: P11279	LC3a Gene Symbol: MAP1LC3A Gene ID: 84557 Accession #: Q9H492	LC3B Gene Symbol: MAP1LC3B MAP1ALC3 Gene ID: 81631 Accession #: Q9GZQ8
NBS1 Gene Symbol: NBN NBS NBS1 P95 Gene ID: 4683 Accession #: O60934	P62 Gene Symbol: SQSTM1 ORCA OSIL Gene ID: 8878 Accession #: Q13501	RHEB Gene Symbol: RHEB RHEB2 Gene ID: 6009 Accession #: Q15382	MSK1 Gene Symbol: RPS6KA5 MSK1 Gene ID: 9252 Accession #: O75582	Alpha-synuclein Gene Symbol: SNCA NACP PARK1 Gene ID: 6622 Accession #: P37840

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis