Human Atherosclerosis Array (20 targets) (SARB0024)

SKU:
SARB0024
€543 - €1,075

Description

Human Atherosclerosis Array Product Information

Product Sku:SARB0024
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:20
Gene Symbols:CCL11, CCL2, CCL20, CCL5, CSF1, CSF2, CSF3, GDNF, ICAM1, IFNG|IL1A, IL1B, IL1R1, IL1R2, LTA, PDGFB, TGFB1, TGFB2, TGFB3, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:MAPK Signaling / NFkB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Atherosclerosis Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Gene ID: 6356
  • Accession #: P51671
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
ICAM-1 (CD54)
  • Gene Symbol: ICAM1
  • Gene ID: 3383
  • Accession #: P05362
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
IL-1 R1
  • Gene Symbol: IL1R1
  • Gene ID: 3554
  • Accession #: P14778
IL-1 R2
  • Gene Symbol: IL1R2
  • Gene ID: 7850
  • Accession #: P27930
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
  

Human Atherosclerosis Array Kit Components

  • Human Atherosclerosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Atherosclerosis Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Eotaxin-1 (CCL11)
Target:
GCSF
Target:
GDNF
Target:
GM-CSF
Target:
ICAM-1 (CD54)
Target:
IFN-gamma
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-1 R1
Target:
IL-1 R2
Target:
MCP-1 (CCL2)
Target:
M-CSF
Target:
TGF beta 1
Target:
TGF beta 2
Target:
TGF beta 3
Target:
TNF alpha
Target:
TNF beta (TNFSF1B)
Target:
MIP-3 alpha (CCL20)
Target:
PDGF-BB
Target:
RANTES (CCL5)
Reactivity:
Human
Application:
Multiplex Array
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