Description
Human Atherosclerosis Array (20 targets) (SARB0024)
The Human Atherosclerosis Array (SARB0024) from Assay Genie is a comprehensive research tool designed to analyze 20 key targets associated with atherosclerosis, a chronic inflammatory disease that affects the arteries. This array allows researchers to simultaneously measure the levels of various proteins involved in the pathogenesis of atherosclerosis, including cytokines, chemokines, and growth factors.With this array, researchers can gain valuable insights into the molecular mechanisms underlying atherosclerosis and identify potential therapeutic targets for the treatment of this disease.
The assay is easy to use, requires a minimal sample volume, and provides accurate and reproducible results, making it an essential tool for studies in cardiovascular research and drug development.By utilizing the Human Atherosclerosis Array from Assay Genie, researchers can accelerate their research efforts and make significant advancements in the understanding and management of atherosclerosis. Order this array today and take a step closer to unraveling the complexities of this prevalent and life-threatening condition.
| Product Sku: | SARB0024 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 20 |
| Gene Symbols: | CCL11, CCL2, CCL20, CCL5, CSF1, CSF2, CSF3, GDNF, ICAM1, IFNG|IL1A, IL1B, IL1R1, IL1R2, LTA, PDGFB, TGFB1, TGFB2, TGFB3, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | MAPK Signaling / NFkB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| GCSF
| GDNF
| GM-CSF
| ICAM-1 (CD54)
|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 R1
| IL-1 R2
|
MCP-1 (CCL2)
| M-CSF
| TGF beta 1
| TGF beta 2
| TGF beta 3
|
TNF alpha
| TNF beta (TNFSF1B)
| MIP-3 alpha (CCL20)
| PDGF-BB
| RANTES (CCL5)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Atherosclerosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
