Human Cytokine Array (49 targets) (SARB0032)

SKU:
SARB0032
€888 - €1,832

Description

Human Cytokine Array Product Information

Product Sku:SARB0032
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:49
Gene Symbols:ACE2, AFP, ANGPT1, ANGPTL4, B2M, CA (19-9), CA9, CALCA, CCL14, CCL21|CD40LG, CDH1, CEACAM1, CEACAM5, CGA, CGB, CRP, CTSS, DCN, DKK1, DKK3, DKK4, DPP4, EDA, EPCAM, EPOR, ERBB2, FCER2, FSHB, HBEGF, IL13RA1, IL17B, IL17C, IL17F, IL17RA, KLK3, MUC1, MUC16, PARN, PLG, PROK1, RPS27A, S100B, SHH, TDGF1, TG, TGFBI, TNFRSF14, TNFRSF17, TNFRSF9, TNFSF9
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

4-1BB (TNFRSF9/CD137)ACE-2Alpha-fetoproteinAngiopoietin-1Angiostatin
ANGPTL4Beta-2 MicroglobulinBCMA (TNFRSF17)beta IG-H3CA125
CA15-3CA19-9CA9Cathepsin SHCC-1 (CCL14)
6Ckine (CCL21)CD23CD40 Ligand (TNFSF5)CEACEACAM-1
Cripto-1CRP (C-Reactive Protein)DANDecorinDKK-1
Dkk-3Dkk-4CD26 (DPPIV)E-CadherinEDA-A2
EG-VEGF (PK1)TROP1 (EpCAM)ErbB2Erythropoietin RFSH
HB-EGFhCG intactHVEM (TNFRSF14)IL-13 R1IL-17B
IL-17CIL-17FIL-17 RAProcalcitoninPSA-free
S100 BSonic Hedgehog N-Terminal (Shh-N)ThyroglobulinUbiquitin+1 

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C10 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
4-1BB (TNFRSF9/CD137)
Target:
ACE-2
Target:
Alpha-fetoprotein
Target:
Angiopoietin-1
Target:
Angiostatin
Target:
ANGPTL4
Target:
Beta-2 Microglobulin
Target:
BCMA (TNFRSF17)
Target:
beta IG-H3
Target:
CA125
Target:
CA15-3
Target:
CA19-9
Target:
CA9
Target:
Cathepsin S
Target:
HCC-1 (CCL14)
Target:
6Ckine (CCL21)
Target:
CD23
Target:
CD40 Ligand (TNFSF5)
Target:
CEA
Target:
CEACAM-1
Target:
Cripto-1
Target:
CRP (C-Reactive Protein)
Target:
DAN
Target:
Decorin
Target:
DKK-1
Target:
Dkk-3
Target:
Dkk-4
Target:
CD26 (DPPIV)
Target:
E-Cadherin
Target:
EDA-A2
Target:
EG-VEGF (PK1)
Target:
TROP1 (EpCAM)
Target:
ErbB2
Target:
Erythropoietin R
Target:
FSH
Target:
HB-EGF
Target:
hCG intact
Target:
HVEM (TNFRSF14)
Target:
IL-13 R1
Target:
IL-17B
Target:
IL-17C
Target:
IL-17F
Target:
IL-17 RA
Target:
Procalcitonin
Reactivity:
Human
Application:
Multiplex Array
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