Human MMP Array (10 targets) (SARB0056)

SKU:
SARB0056
€531 - €1,047

Description

Human MMP Array Product Information

Product Sku:SARB0056
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:10
Gene Symbols:MMP1, MMP10, MMP13, MMP2, MMP3, MMP8, MMP9, TIMP1, TIMP2, TIMP4
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / HIF-1 alpha Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human MMP Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

MMP-1
  • Gene Symbol: MMP1
  • Gene ID: 4312
  • Accession #: P03956
MMP-2
  • Gene Symbol: MMP2
  • Gene ID: 4313
  • Accession #: P08253
MMP-3
  • Gene Symbol: MMP3
  • Gene ID: 4314
  • Accession #: P08254
MMP-8
  • Gene Symbol: MMP8
  • Gene ID: 4317
  • Accession #: P22894
MMP-9
  • Gene Symbol: MMP9
  • Gene ID: 4318
  • Accession #: P14780
MMP-10
  • Gene Symbol: MMP10
  • Gene ID: 4319
  • Accession #: P09238
MMP-13
  • Gene Symbol: MMP13
  • Gene ID: 4322
  • Accession #: P45452
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: 7076
  • Accession #: P01033
TIMP-2
  • Gene Symbol: TIMP2
  • Gene ID: 7077
  • Accession #: P16035
TIMP-4
  • Gene Symbol: TIMP4
  • Gene ID: 7079
  • Accession #: Q99727

Human MMP Array Kit Components

  • Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human MMP Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
MMP-1
Target:
MMP-2
Target:
MMP-3
Target:
MMP-8
Target:
MMP-9
Target:
MMP-10
Target:
MMP-13
Target:
TIMP-1
Target:
TIMP-2
Target:
TIMP-4
Reactivity:
Human
Application:
Multiplex Array
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