Human Cancer Discovery Array (10 targets) (SARB0027)

SKU:
SARB0027
€497 - €973

Description

Human Cancer Discovery Array Product Information

Product Sku:SARB0027
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:10
Gene Symbols:AFP, CA (19-9), CEACAM5, GDF15, HGF, KLK3, MUC16, SPP1, TIMP1, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cancer Discovery Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Alpha-fetoprotein
  • Gene Symbol: AFP HPAFP
  • Gene ID: 174
  • Accession #: P02771
CA125
  • Gene Symbol: MUC16 CA125
  • Gene ID: 94025
  • Accession #: Q8WXI7
CA19-9CEA
  • Gene Symbol: CEACAM5 CEA
  • Gene ID: 1048
  • Accession #: P06731
GDF-15
  • Gene Symbol: GDF15 MIC1 PDF PLAB PTGFB
  • Gene ID: 9518
  • Accession #: Q99988
HGF
  • Gene Symbol: MET
  • Gene ID: 3082
  • Accession #: P14210
Osteopontin (SPP1)
  • Gene Symbol: SPP1 BNSP OPN PSEC0156
  • Gene ID: 6696
  • Accession #: P10451
PSA-free
  • Gene Symbol: KLK3 APS
  • Gene ID: 354
  • Accession #: P07288
TIMP-1
  • Gene Symbol: TIMP1 CLGI TIMP
  • Gene ID: 7076
  • Accession #: P01033
VEGF-A
  • Gene Symbol: VEGFA VEGF
  • Gene ID: 7422
  • Accession #: P15692

Human Cancer Discovery Array Kit Components

  • Human Cancer Discovery Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cancer Discovery Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Alpha-fetoprotein
Target:
CA125
Target:
CA19-9
Target:
CEA
Target:
GDF-15
Target:
HGF
Target:
Osteopontin (SPP1)
Target:
PSA-free
Target:
TIMP-1
Target:
VEGF-A
Reactivity:
Human
Application:
Multiplex Array
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