Human Neuro Discovery Array (20 targets) (SARB0057)

SKU:
SARB0057
€565 - €1,121

Description

Human Neuro Discovery Array Product Information

Product Sku:SARB0057
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:20
Gene Symbols:BDNF, CCL2, CCL3, CSF3, CXCL8, GDNF, HBEGF, IFNG, IGF1, IL10|IL1A, IL1B, IL6, MMP2, MMP3, NGF, S100B, TGFB1, TNF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Neuro Discovery Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

BDNF
  • Gene Symbol: BDNF
  • Gene ID: 627
  • Accession #: P23560
beta-NGF
  • Gene Symbol: NGF NGFB
  • Gene ID: 4803
  • Accession #: P01138
GCSF
  • Gene Symbol: CSF3 C17orf33 GCSF
  • Gene ID: 1440
  • Accession #: P09919
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
HB-EGF
  • Gene Symbol: HBEGF DTR DTS HEGFL
  • Gene ID: 1839
  • Accession #: Q99075
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 3479
  • Accession #: P05019
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
IL-8 (CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 3576
  • Accession #: P10145
MCP-1 (CCL2)
  • Gene Symbol: CCL2 SCYA2
  • Gene ID: 6347
  • Accession #: P13500
  

Human Neuro Discovery Array Kit Components

  • Human Neuro Discovery C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Neuro Discovery Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
BDNF
Target:
beta-NGF
Target:
GCSF
Target:
GDNF
Target:
HB-EGF
Target:
IFN-gamma
Target:
IGF-1
Target:
IL-10
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-6
Target:
IL-8 (CXCL8)
Target:
MCP-1 (CCL2)
Target:
MIP-1 alpha (CCL3)
Target:
MMP-2
Target:
MMP-3
Target:
S100 B
Target:
TGF beta 1
Target:
TNF alpha
Target:
VEGF-A
Reactivity:
Human
Application:
Multiplex Array
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