Human Neuro Discovery Array (20 targets)

Product Sku:	SARB0057
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	20
Gene Symbols:	BDNF, CCL2, CCL3, CSF3, CXCL8, GDNF, HBEGF, IFNG, IGF1, IL10\|IL1A, IL1B, IL6, MMP2, MMP3, NGF, S100B, TGFB1, TNF, VEGFA
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	Akt Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560	beta-NGF Gene Symbol: NGF NGFB Gene ID: 4803 Accession #: P01138	GCSF Gene Symbol: CSF3 C17orf33 GCSF Gene ID: 1440 Accession #: P09919	GDNF Gene Symbol: GDNF Gene ID: 2668 Accession #: P39905	HB-EGF Gene Symbol: HBEGF DTR DTS HEGFL Gene ID: 1839 Accession #: Q99075
IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584
IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231	IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 3576 Accession #: P10145	MCP-1 (CCL2) Gene Symbol: CCL2 SCYA2 Gene ID: 6347 Accession #: P13500	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147	MMP-2 Gene Symbol: MMP2 CLG4A Gene ID: 4313 Accession #: P08253
MMP-3 Gene Symbol: MMP3 STMY1 Gene ID: 4314 Accession #: P08254	S100 B Gene Symbol: S100B Gene ID: 6285 Accession #: P04271	TGF beta 1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 7422 Accession #: P15692

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis