Description
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Mouse Neuro Discovery Array (23 targets) (SARB0091)
The Mouse Neuro Discovery Array 23 Targets (SARB0091) is a powerful tool for studying neurobiology and neuroscience research. This array contains 23 different targets related to neurology, including neurotransmitters, receptors, and proteins involved in synaptic function. With the Mouse Neuro Discovery Array 23 Targets, researchers can simultaneously measure the expression levels of multiple key proteins in the mouse brain, providing valuable insights into the molecular mechanisms underlying neurological disorders, neurodegenerative diseases, and cognitive functions.
This array is easy to use, sensitive, and reproducible, making it an essential tool for researchers studying the intricate workings of the brain. Whether studying synaptic signaling pathways, neurotransmitter receptors, or neuroinflammation markers, the Mouse Neuro Discovery Array 23 Targets provides a comprehensive and detailed analysis of the molecular landscape of the mouse brain.
| Product Sku: | SARB0091 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 23 |
| Gene Symbols: | AGER, CCL17, CCL2, CCL3, CSF1, CSF3, CX3CL1, CXCL1, CXCL12, CXCL5|FASLG, IFNG, IGF1, IL10, IL1A, IL1B, IL4, IL6, MMP2, MMP3, TGFB1, TNF, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Fas Ligand (TNFSF6)
| Fractalkine (CX3CL1)
| GCSF
| IFN-gamma
| IGF-1
|
IL-10
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-4
| IL-6
|
KC (CXCL1)
| LIX
| MCP-1 (CCL2)
| M-CSF
| MIP-1 alpha (CCL3)
|
MMP-2
| MMP-3
| RAGE
| TARC (CCL17)
| SDF-1 alpha (CXCL12 alpha)
|
TGF beta 1
| TNF alpha
| VEGF-A
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Neuro Discovery C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
