Human Neuro Discovery Array (30 targets) (SARB0058)

SKU:
SARB0058
€629 - €1,264

Description

Human Neuro Discovery Array Product Information

Product Sku:SARB0058
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:30
Gene Symbols:ADIPOQ, BDNF, CCL11, CCL17, CCL2, CCL24, CCL26, CCL4, CNTF, CRP|CSF2, CXCL8, FAS, GDNF, IFNG, IL10, IL18, IL1A, IL1B, IL4, IL6, LIF, MMP2, MMP3, NGF, S100B, TGFB1, TIMP1, TNF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Neuro Discovery Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Adiponectin (ACRP30)
  • Gene Symbol: ADIPOQ ACDC ACRP30 APM1 GBP28
  • Gene ID: 9370
  • Accession #: Q15848
BDNF
  • Gene Symbol: BDNF
  • Gene ID: 627
  • Accession #: P23560
beta-NGF
  • Gene Symbol: NGF NGFB
  • Gene ID: 4803
  • Accession #: P01138
CNTF
  • Gene Symbol: CNTF
  • Gene ID: 1270
  • Accession #: P26441
CRP (C-Reactive Protein)
  • Gene Symbol: CRP PTX1
  • Gene ID: 1401
  • Accession #: P02741
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11 SCYA11
  • Gene ID: 6356
  • Accession #: P51671
Eotaxin-2 (MPIF-2/CCL24)
  • Gene Symbol: CCL24 MPIF2 SCYA24
  • Gene ID: 6369
  • Accession #: O00175
Eotaxin-3 (CCL26)
  • Gene Symbol: CCL26 SCYA26 UNQ216/PRO242
  • Gene ID: 10344
  • Accession #: Q9Y258
Fas (TNFRSF6/Apo-1)
  • Gene Symbol: FASLG FASL TNFSF6
  • Gene ID: 355
  • Accession #: P25445
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
  

Human Neuro Discovery Array Kit Components

  • Human Neuro Discovery C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Neuro Discovery Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Adiponectin (ACRP30)
Target:
BDNF
Target:
beta-NGF
Target:
CNTF
Target:
CRP (C-Reactive Protein)
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
Eotaxin-3 (CCL26)
Target:
Fas (TNFRSF6/Apo-1)
Target:
IL-4
Target:
GDNF
Target:
GM-CSF
Target:
IFN-gamma
Target:
IL-10
Target:
IL-18
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-6
Target:
IL-8 (CXCL8)
Target:
LIF
Target:
MCP-1 (CCL2)
Target:
MIP-1 beta (CCL4)
Target:
MMP-2
Target:
MMP-3
Target:
S100 B
Target:
TARC (CCL17)
Target:
TGF beta 1
Target:
TIMP-1
Target:
TNF alpha
Target:
VEGF-A
Reactivity:
Human
Application:
Multiplex Array
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