Human Neuro Discovery Array (30 targets) (SARB0058)
- SKU:
- SARB0058
- Product Type:
- Protein Array
- Analytes:
- Adiponectin (ACRP30)
- BDNF
- beta-NGF
- CNTF
- CRP (C-Reactive Protein)
- Eotaxin-1 (CCL11)
- Eotaxin-2 (MPIF-2/CCL24)
- Eotaxin-3 (CCL26)
- Fas (TNFRSF6/Apo-1)
- IL-4
- GDNF
- GM-CSF
- IFN-gamma
- IL-10
- IL-18
- IL-1 alpha (IL-1 F1)
- IL-1 beta (IL-1 F2)
- IL-6
- IL-8 (CXCL8)
- LIF
- MCP-1 (CCL2)
- MIP-1 beta (CCL4)
- MMP-2
- MMP-3
- S100 B
- TARC (CCL17)
- TGF beta 1
- TIMP-1
- TNF alpha
- VEGF-A
- Reactivity:
- Human
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
Product Sku: | SARB0058 |
Size: | 2, 4, or 8 |
Species Detected: | Human |
Number of Targets Detected: | 30 |
Gene Symbols: | ADIPOQ, BDNF, CCL11, CCL17, CCL2, CCL24, CCL26, CCL4, CNTF, CRP|CSF2, CXCL8, FAS, GDNF, IFNG, IL10, IL18, IL1A, IL1B, IL4, IL6, LIF, MMP2, MMP3, NGF, S100B, TGFB1, TIMP1, TNF, VEGFA |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
Adiponectin (ACRP30)
| BDNF
| beta-NGF
| CNTF
| CRP (C-Reactive Protein)
|
Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| Fas (TNFRSF6/Apo-1)
| IL-4
|
GDNF
| GM-CSF
| IFN-gamma
| IL-10
| IL-18
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-6
| IL-8 (CXCL8)
| LIF
|
MCP-1 (CCL2)
| MIP-1 beta (CCL4)
| MMP-2
| MMP-3
| S100 B
|
TARC (CCL17)
| TGF beta 1
| TIMP-1
| TNF alpha
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Neuro Discovery C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Biotinylated Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated Streptavidin |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |