Description
Human MMP Array (10 targets) (SARB0056)
The Human MMP Array (10 Targets) (SARB0056) from AssayGenie is a powerful tool for studying matrix metalloproteinases (MMPs), key enzymes involved in tissue remodeling, wound healing, and cancer progression. This array allows for the simultaneous detection of 10 different MMP targets in human samples, providing valuable insights into the regulation and activity of these important proteins.The array is designed for high sensitivity and specificity, with robust performance in a variety of sample types.
It is suitable for use in both research and clinical settings, enabling researchers to explore the role of MMPs in various disease states and therapeutic interventions.With the Human MMP Array (10 Targets) (SARB0056), researchers can accelerate their studies on MMP biology and develop novel approaches for diagnosing and treating diseases associated with MMP dysregulation. This versatile tool is a must-have for any laboratory investigating the complex roles of MMPs in health and disease.
| Product Sku: | SARB0056 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 10 |
| Gene Symbols: | MMP1, MMP10, MMP13, MMP2, MMP3, MMP8, MMP9, TIMP1, TIMP2, TIMP4 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / HIF-1 alpha Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
MMP-1
| MMP-2
| MMP-3
| MMP-8
| MMP-9
|
MMP-10
| MMP-13
| TIMP-1
| TIMP-2
| TIMP-4
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
