Human Th1/Th2/Th17 Array (34 targets) (SARB0071)

SKU:
SARB0071
€644 - €1,297

Description

Human Th1/Th2/Th17 Array Product Information

Product Sku:SARB0071
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:34
Gene Symbols:CCL20, CD40, CD40LG, CSF2, CSF3, IFNG, IFNL2, IL10, IL12A, IL12B|IL13, IL17A, IL17F, IL17RA, IL1B, IL1R1, IL1R2, IL2, IL21, IL21R, IL22, IL23A, IL4, IL5, IL6, IL6R, IL6ST, LTA, TGFB1, TGFB3, TNF, TNFRSF18, TNFRSF8, TNFSF11
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:NFkB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Th1/Th2/Th17 Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

CD30 (TNFRSF8)CD40 Ligand (TNFSF5)CD40 (TNFRSF5)GCSFGITR (TNFRSF18)
GM-CSFIFN-gammaIL-1 beta (IL-1 F2)IL-1 R1IL-1 R2
IL-2IL-4IL-5IL-6IL-6 R
IL-10IL-12 p40IL-12 p70IL-13IL-17A
IL-17FIL-17 RAIL-21IL-21 RIL-22
IL-23IL-28A (IFN-lambda 2)MIP-3 alpha (CCL20)gp130TGF beta 1
TGF beta 3TNF alphaTNF beta (TNFSF1B)TRANCE (TNFSF11) 

Human Th1/Th2/Th17 Array Kit Components

  • Human Th1/Th2/Th17 Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Th1/Th2/Th17 Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
CD30 (TNFRSF8)
Target:
CD40 Ligand (TNFSF5)
Target:
CD40 (TNFRSF5)
Target:
GCSF
Target:
GITR (TNFRSF18)
Target:
GM-CSF
Target:
IFN-gamma
Target:
IL-1 beta (IL-1 F2)
Target:
IL-1 R1
Target:
IL-1 R2
Target:
IL-2
Target:
IL-4
Target:
IL-5
Target:
IL-6
Target:
IL-6 R
Target:
IL-10
Target:
IL-12 p40
Target:
IL-12 p70
Target:
IL-13
Target:
IL-17A
Target:
IL-17F
Target:
IL-17 RA
Target:
IL-21
Target:
IL-21 R
Target:
IL-22
Target:
IL-23
Target:
IL-28A (IFN-lambda 2)
Target:
MIP-3 alpha (CCL20)
Target:
gp130
Target:
TGF beta 1
Target:
TGF beta 3
Target:
TNF alpha
Target:
TNF beta (TNFSF1B)
Target:
TRANCE (TNFSF11)
Reactivity:
Human
Application:
Multiplex Array
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