GeniePlex Human Th1/Th2/Th17/Treg 10-Plex

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ELISA Kit Technical Manual

Product Name: GeniePlex Human Th1/Th2/Th17/Treg 10-Plex Immunoassay Panel


G-CSF, GM-CSF, IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha and TNF-Beta

Species Reactivity:


Assay Type:


Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex GeniePlex Human Th1/Th2/Th17/Treg 10-Plex Immunoassay Panel is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes G-CSF, GM-CSF, IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha and TNF-Beta on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 10 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Analytes:G-CSF, GM-CSF, IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha and TNF-Beta
1x Ab-conjugated beads (premixed):S4P3 - anti-H IL-10; S4P4 - anti-H IL-6; S4P6 - anti-H TNFalpha; S4P7 - anti-H IL-2; S4P11 - anti-H TNFbeta; S5P2 - anti-H IFNgamma; S5P3 - anti-H GM-CSF; S5P4 - anti-H IL-4; S5P7 - anti-H IL-17A; S5P10 - anti-H G-CSF
2x Biotin-detection Ab (premixed):Biotin - anti-H IL-10; Biotin - anti-H IL-6; Biotin - anti-H TNFalpha; Biotin - anti-H IL-2; Biotin - anti-H TNFbeta; Biotin - anti-H IFNgamma; Biotin - anti-H GM-CSF; Biotin - anti-H IL-4; Biotin - anti-H IL-17A; Biotin - anti-H G-CSF
Buffers:2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix-Human TH1/Th2 10-Plex. One vial containing lyophilized recombinant G-CSF, GM-CSF, IFNgamma, IL-10, IL-17A, IL-2, IL-4, IL-6, TNFalpha and TNFbeta.
Sample types:Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs):G-CSF, < 5 pg/mL; GM-CSF, < 5 pg/mL; IFN-Gamma, < 3 pg/mL; IL-2, < 3 pg/mL; IL-4, < 1 pg/mL; IL-6, < 5 pg/mL; IL-10, < 2 pg/mL; IL-17A, < 3 pg/mL; TNF-Alpha, < 1 pg/mL; TNF-Beta, < 5 pg/mL.
LLOQs:G-CSF, < 10 pg/mL; GM-CSF, < 10 pg/mL; IFN-Gamma, < 10 pg/mL; IL-2, < 10 pg/mL; IL-4, < 3 pg/mL; IL-6, < 10 pg/mL; IL-10, < 5 pg/mL; IL-17A, < 10 pg/mL; TNF-Alpha, < 20pg/mL; TNF-Beta, < 10 pg/mL.
ULOQs:G-CSF, > 5,000 pg/mL; GM-CSF, > 5,000 pg/mL; IFN-Gamma, > 2,000 pg/mL; IL-2, > 2,000 pg/mL; IL-4, > 1,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 5,000 pg/mL; IL-17A, > 1,000 pg/mL; TNF-Alpha, > 1,000 pg/mL; TNF-Beta, > 5,000 pg/mL..
Standard dose recovery:Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: < 10%
Inter-assay CV:Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume:Sample volume: 15 µL/test


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